Application of high-performance capillary electrophoresis to analysis of carbohydrates, especially those in glycoproteins
Application of high-performance capillary electrophoresis to analysis of carbohydrates, especially those in glycoproteins
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DOI：
10.1016/1046-2023(92)90038-a
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发表时间：
1992-01-01
期刊：
影响因子：
--
通讯作者：
Kakehi, Kazuaki
中科院分区：
文献类型：
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作者：
Honda, Susumu;Suzuki, Shigeo;Kakehi, Kazuaki
本文介绍了基于还原性吡啶胺化衍生物的高效毛细管电泳（HPCE）的单糖组分分析和糖蛋白研究中的寡糖图谱分析方法。将糖蛋白酸水解释放的单糖组分转化为PA衍生物，并以硼酸盐络合物的形式通过HPCE进行分析。它们可以在皮摩尔范围（引入量）内以高再现性进行定量。肼解释放的寡糖类似地转化为PA衍生物。这些衍生物的相对迁移率，在酸性磷酸盐缓冲液和碱性硼酸盐缓冲液中获得的二维映射，确保可靠的鉴定的寡糖。
Methods for the component monosaccharide analysis and oligosaccharide mapping for glycoprotein research, based on HPCE of reductively pyridylaminated (PA) derivatives, are described. The component monosaccharides released from glycoproteins by acid hydrolysis are converted to PA derivatives and analyzed by HPCE as borate complexes. They can be quantified in the picomole range (introduced amount) with high reproducibility. The oligosaccharides released by hydrazinolysis are similarly converted to PA derivatives. Two-dimensional mapping of the relative mobilities of these derivatives, obtained in an acidic phosphate buffer and an alkaline borate buffer, ensures reliable identification of the oligosaccharides.