Influence of EDC/NHS coupling chemistry on stability and cytotoxicity of ZnO nanoparticles modified with proteins
Influence of EDC/NHS coupling chemistry on stability and cytotoxicity of ZnO nanoparticles modified with proteins
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DOI：
10.1016/j.apsusc.2017.01.235
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发表时间：
2017-05-01
影响因子：
6.7
通讯作者：
Culha, Mustafa
中科院分区：
文献类型：
--
作者：
Kelestemur, Seda;Altunbek, Mine;Culha, Mustafa
ZnO纳米粒子（NPs）的毒性越来越受到关注，这是由于其在包括防晒剂、油漆、颜料和陶瓷在内的多种产品中的抗菌、抗真菌、抗腐蚀和紫外线过滤特性而增加使用。ZnO纳米颗粒的毒性主要归因于Zn 2+释放导致细胞内活性氧（ROS）水平增加。用生物相容性配体或聚合物进行表面改性可以是降低基于溶解的毒性的良好策略。在两项先前的研究中，报道了ZnO NP的EDC/NHS偶联化学的矛盾结果。在这项研究中，相同的表面改性策略，强调ZnO纳米颗粒的稳定性澄清。首先，用过氧化氢（H2 O2）处理增加ZnO NPs上的OH基团的密度，然后在ZnO NPs（Si-ZnO）表面上进行二氧化硅涂覆。最后，牛血清白蛋白（BSA）在三个不同浓度的ZnO-Si的共价连接进行EDC/NHS耦合化学。ZnO NP在EDC/NHS偶联化学的酸性条件下具有非常高的溶解速率，如由ICP-MS分析所确定的。此外，偶联反应中ZnO纳米粒子的用量对Zn ~（2+）的溶解速率有重要影响，并依赖于ZnO纳米粒子表面附着的BSA。最后，评价了BSA修饰的Si-ZnO纳米颗粒对人肺癌（A549）和人皮肤成纤维细胞（HSF）的细胞毒性。虽然观察到BSA修饰的ZnO纳米颗粒与细胞的结合增加，但修饰显著降低了它们的细胞毒性。这可以用具有表面改性的ZnO NPs的活性表面积减小来解释。然而，增加线粒体去极化和ROS的生产被观察到取决于BSA的覆盖量。(C)2017爱思唯尔B. V.保留所有权利。
The toxicity of ZnO nanoparticles (NPs) is a growing concern due to its increasing use in several products including sunscreens, paints, pigments and ceramics for its antibacterial, antifungal, anti-corrosive and UV filtering properties. The toxicity of ZnO NPs is mostly attributed to the Zn2+ release causing an increase in the intracellular reactive oxygen species (ROS) level. The surface modification with a biocompatible ligand or a polymer can be a good strategy to reduce dissolution based toxicity. In two previous studies, the conflicting results with EDC/NHS coupling chemistry for ZnO NPs were reported. In this study, the same surface modification strategy with an emphasis on the stability of ZnO NPs is clarified. First, the density of OH groups on the ZnO NPs is increased with hydrogen peroxide (H2O2) treatment, and then a silica coating on the ZnO NPs (Si-ZnO) surface is performed. Finally, a covalent attachment of bovine serum albumin (BSA) on three different concentrations of ZnO-Si is carried out by EDC/NHS coupling chemistry. ZnO NPs have a very high dissolution rate under acidic conditions of EDC/NHS coupling chemistry as determined from the ICP-MS analysis. In addition, the amount of ZnO NPs in coupling reaction has an important effect on the dissolution rate of Zn2+ and dependently BSA attached on the ZnO NP surfaces. Finally, the cytotoxicity of the BSA modified Si-ZnO NPs on human lung cancer (A549) and human skin fibroblast (HSF) is evaluated. Although an increased association of BSA modified ZnO NPs with cells was observed, the modification significantly decreased their cytotoxicity. This can be explained with the decreased active surface area of ZnO NPs with the surface modification. However, an increase in the mitochondrial depolarization and ROS production was observed depending on the amount of BSA coverage. (C) 2017 Elsevier B.V. All rights reserved.