Optimum selection of extraction methods of extracellular polymeric substances in activated sludge for effective extraction of the target components
Optimum selection of extraction methods of extracellular polymeric substances in activated sludge for effective extraction of the target components
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DOI：
10.1016/j.bej.2017.08.002
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发表时间：
2017-11-15
影响因子：
3.9
通讯作者：
Ito, Tomohiro
中科院分区：
文献类型：
--
作者：
Phuc-Nguon Hong;Honda, Ryo;Ito, Tomohiro
研究了活性污泥胞外聚合物（EPS）提取方法对提取效率和提取液组成的影响。基于蛋白质和多糖组成、激发-发射矩阵（EEM）和分子量（MW）分布，对EPS提取物进行了表征。采用4种方法提取活性污泥中的EPS：甲醛与氢氧化钠结合法、氢氧化钠与加热结合法、阳离子交换树脂法（CER）提取紧密结合的EPS、超声法提取松散结合的EPS。提取方法不仅影响提取效率，而且影响提取的EPS的蛋白质和多糖组成、EEM光谱和MW谱。甲醛和碱的组合是最好的，以获得高的提取效率，细胞裂解少。EEM分析后，CER方法是适当的，以检测范围广泛的荧光有机物质，而碱提取是有效的检测腐殖酸类物质。碱抽提法更适合于各种分子量的大分子EPS的抽提。然而，应该注意的是，在碱提取过程中可能会发生MW分布的变化。因此，提取方法的选择是实现EPS目标成分有效提取的关键。(C)2017爱思唯尔B. V.保留所有权利。
Effects of extraction methods of extracellular polymeric substances (EPS) from activated sludge on extraction efficiency and extract composition were investigated. EPS extract was characterized based on protein and polysaccharide composition, excitation-emission matrix (EEM) and molecular weight (MW) profile. Four methods were applied to extract EPS from activated sludge: combination of formaldehyde and sodium hydroxide, combination of sodium hydroxide and heating, cation exchange resin (CER) for tightly-bound EPS, and sonication for loosely-bound EPS. Extraction methods affected not only extraction efficiency but also composition of proteins and polysaccharides, EEM spectra and MW profile of extracted EPS. Combination of formaldehyde and alkaline is preferable to acquire high extraction efficiency with less cell lysis. Upon EEM analysis, the CER method was appropriate to detect a wide range of fluorescent organic substances, while alkaline extraction was less efficient for detection of humic acid-like substances. Alkaline extraction is more appropriate for extraction of large EPS molecules with a variety of MWs. However, it should be noted that changes in MW distribution may occur during alkaline extraction. Consequently, selection of extraction methods is crucial to achieving effective extraction of the target components of EPS. (C) 2017 Elsevier B.V. All rights reserved.