Tea catechins inhibit angiogenesis endothelial cell growth, migration in vitro, measured by human and tube formation, through inhibition of VEGF receptor binding
Tea catechins inhibit angiogenesis endothelial cell growth, migration in vitro, measured by human and tube formation, through inhibition of VEGF receptor binding
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DOI：
10.1016/s0304-3835(02)00007-1
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发表时间：
2002-06-28
期刊：
影响因子：
9.7
通讯作者：
Kaji, K
中科院分区：
文献类型：
--
作者：
Kondo, T;Ohta, T;Kaji, K
我们研究了茶儿茶素（EC, ECg， EGC, EGCg）是否对血管生成有抑制作用，以及它们在血管生成过程中的哪个阶段起作用。儿茶素对体外血管生成模型的影响，即人脐静脉内皮细胞的生长、迁移和成管。在三种不同的浓度范围为1.56至100 muM的生物测定中，所有四种儿茶素都能抑制体外血管生成。在四种儿茶素测试中，表没食子儿茶素没食子酸酯（EGCg）在所有三种检测中抑制血管生成最有效。四种儿茶素在VEGF结合实验中，只有EGCg抑制血管生成主要诱导因子VEGF与内皮细胞的结合，且呈浓度依赖性。这些结果表明，虽然所有四种茶儿茶素都抑制血管生成过程，但EGCg单独可以减少VEGF与其受体的结合，从而影响下游信号传导。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
We have investigated whether tea catechins EC, ECg, EGC, EGCg) have any inhibitory effects on angiogenesis and which step they affect during the process. The effects of catechins were tested on in vitro models of angiogenesis, namely, growth, migration and tube formation of human umbilical vein endothelial cells. All four catechins inhibited angiogenesis in vitro in the three different bioassays with concentrations ranging from 1.56 to 100 muM. Among the four catechins tested, epigallocatechin gallate (EGCg) was the most effective in inhibiting angiogenesis in all three assays. When these four catechins were tested on VEGF binding assay, only EGCg inhibited the binding of VEGF, a major angiogenesis inducing factor, to endothelial cells in a concentration dependent manner. These results indicate that while all four tea catechins inhibit the process of angiogenesis, EGCg alone can reduce the binding of VEGF to its receptors and thus affects the downstream signaling. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.