Noninvasive measurement of the refractive index of cell organelles using surface plasmon resonance microscopy
Noninvasive measurement of the refractive index of cell organelles using surface plasmon resonance microscopy
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DOI：
10.1364/ol.44.001359
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发表时间：
2019-03-15
期刊：
影响因子：
3.6
通讯作者：
Kreysing, Eva
中科院分区：
文献类型：
--
作者：
Hassani, Hossein;Kreysing, Eva
真核细胞的健康取决于其细胞器的正常功能。在不干扰细胞的情况下表征这些纳米到微米尺度的特化亚基是具有挑战性的，但也可以提供有关细胞状态的有价值的见解。我们表明，基于物镜的扫描表面等离子体共振显微镜可用于定量分析细胞器的折射率，以无创和无标记的方式，在衍射极限具有横向分辨率。(C) 2019美国光学学会
The health of a eukaryotic cell depends on the proper functioning of its cell organelles. Characterizing these nanometer-to micrometer-scaled specialized subunits without disturbing the cell is challenging but can also provide valuable insights regarding the state of a cell. We show that objective-based scanning surface plasmon resonance microscopy can be used to analyze the refractive index of cell organelles quantitatively in a noninvasive and label-free manner with a lateral resolution at the diffraction limit. (C) 2019 Optical Society of America