Neural and hormonal mechanisms mediate the enterogastric reflex: a study in intestinal transplants in rats.
Neural and hormonal mechanisms mediate the enterogastric reflex: a study in intestinal transplants in rats.
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神经和激素机制介导肠胃反射：大鼠肠道移植的一项研究。
DOI：
10.1016/0016-5085(91)90533-q
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发表时间：
1991
期刊：
影响因子：
29.4
通讯作者：
Debas,HT
中科院分区：
文献类型：
--
作者：
Orloff,SL;Bunnett,NW;Wong,H;Walsh,JH;Debas,HT
为了确定神经反射和肠道激素对肠道脂肪抑制胃酸分泌的相对贡献，研究了具有外源性去神经移植空肠段的大鼠和具有神经支配的空肠段的大鼠。术后，通过胃外滴定法测定餐后胃酸分泌。当分泌达到平台时，将分级剂量的油酸或盐水滴入空肠段。在两组中，空肠内脂肪抑制酸分泌，但盐水不抑制酸分泌。在0.4和0.08 mmol油酸的剂量下，有一个25%和17%的最大抑制高原酸反应的神经支配的大鼠比移植大鼠，大概是因为神经的贡献。为了检查激素介质，在移植大鼠中研究了生长抑素单克隆抗体和胆囊收缩素A受体拮抗剂（L-364，718）对脂肪诱导的胃酸分泌抑制的影响。用生长抑素单克隆抗体（2.18 mg IV）或L-364，718（1 mg/kg IV）治疗移植患者，分别使脂肪诱导的酸分泌抑制降低了95%和28%。总之，神经和激素机制介导脂肪诱导的胃酸分泌抑制，激素机制占主导地位。生长抑素和胆囊收缩素在较小程度上有助于激素机制。
To determine the relative contributions of neural reflexes and intestinal hormones to the inhibition of gastric acid secretion by intestinal fat, rats with an extrinsically denervated, transplanted segment of jejunum and those with an innervated segment of jejunum were studied. Postoperatively, meal-stimulated gastric acid secretion was measured by extragastric titration. When secretion reached a plateau, graded doses of oleic acid or saline were instilled into the jejunal segments. In both groups, acid secretion was inhibited by intrajejunal fat but not saline. At doses of 0.4 and 0.08 mmol oleic acid, there was a 25% and 17% greater maximal inhibition of plateau acid response in the innervated rats than in the transplanted rats, presumably because of the neural contribution. To examine the hormonal mediators, the effects of a somatostatin monoclonal antibody and a cholecystokinin A receptor antagonist (L-364,718) on fat-induced inhibition of gastric acid secretion were studied in transplanted rats. Treatment of the patients with transplants with a somatostatin monoclonal antibody (2.18 mg IV) or L-364,718 (1 mg/kg IV) reduced the fat-induced inhibition of acid secretion by 95% and 28%, respectively. In conclusion, both neural and hormonal mechanisms mediate fat-induced inhibition of gastric acid secretion, with the hormonal mechanism predominating. Somatostatin, and to a lesser extent cholecystokinin, contribute to the hormonal mechanism.