Establishment and characterization of renal progenitor like cells from S3 segment of nephron in rat adult kidney
Establishment and characterization of renal progenitor like cells from S3 segment of nephron in rat adult kidney
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DOI：
10.1096/fj.05-3942com
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发表时间：
2005-11-01
期刊：
影响因子：
4.8
通讯作者：
Makino, H
中科院分区：
文献类型：
--
作者：
Kitamura, S;Yamasaki, Y;Makino, H
肾脏被认为是一个再生器官，可以修复急性肾小管损伤。目前尚不清楚细胞群是否有助于修复紊乱的肾脏。我们试图从单个细胞中鉴定和分离高度增殖的细胞。我们从成年大鼠肾脏中解剖出一个肾单位。将分离的肾单位分成片段并培养。在有限稀释后重新铺板长出的细胞，使得每个孔含有单个细胞。其中一个生长能力最强的细胞系被命名为rKS 56。rKS 56细胞显示鹅卵石外观，并表达与肾脏发育相关的未成熟细胞标记物和成熟肾小管细胞标记物。rKS 56细胞呈指数生长，可维持300天而不转化。在不同的培养条件下，rKS 56细胞分化为成熟的肾小管细胞定义的水通道蛋白-1，2的表达，并对甲状旁腺激素或加压素的反应。移植到大鼠缺血再灌注模型的肾脏，移植后rKS 56细胞部分替代受损的肾小管，改善肾功能。这些结果提示rKS 56细胞具有自我更新、多向可塑性和组织修复能力。rKS 56可能有助于肾再生细胞治疗的未来发展。
Kidney is thought to be a regenerative organ in terms of repair from acute tubular injury. It is unknown whether cell population contributes to repair disordered kidney. We attempted to identify and isolate highly proliferative cells from a single cell. We dissected a single nephron from adult rat kidney. Isolated nephrons were separated into segments and cultured. Outgrowing cells were replated after limiting dilution so that each well contained a single cell. One of cell line which was the most potent to grow was designated as rKS56. rKS56 cells showed cobblestone appearance and expressed immature cell markers relating to kidney development and mature tubular cell markers. rKS56 cells grew exponentially and could be maintained for 300 days without transformation. In different culture conditions, rKS56 cells differentiated into mature tubular cells defined by aquaporin-1, 2 expression, and responsiveness to parathyroid hormone or vasopressin. Engrafted to kidney in rat ischemic reperfusion model, rKS56 cells replaced in injured tubules in part after implantation and improved renal function. These results suggest rKS56 cells possess character such as self-renewal, multi-plasticity and capability of tissue repair. rKS56 may possibly contribute to the future development of cell therapy for renal regeneration.