Development of a new biosensor for superoxide radicals
Development of a new biosensor for superoxide radicals
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DOI：
10.1007/s00216-005-0102-x
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发表时间：
2005-11-01
影响因子：
4.3
通讯作者：
Emregül, E
中科院分区：
文献类型：
--
作者：
Emregül, E
通过将超氧化物歧化酶固定在铂电极表面的明胶 (G) 内，开发了一种用于测定超氧自由基的超氧化物歧化酶 (SOD) 生物传感器。研究了生物传感器的特性并确定了最佳条件——酶浓度、戊二醛浓度和pH值。 G-SOD生物传感器的响应与O-2-（中心点-）浓度成正比，在信噪比为3时，检测限为0.01 mmol L-1。使用三周和四周后，生物传感器分别保留了89%和60%的灵敏度。 SOD 固定在明胶上为酶周围提供了生物相容性微环境，并非常有效地稳定了酶的活性。超氧化物歧化酶生物传感器用于测定乙酰水杨酸类药物的抗氧化特性以及健康和癌性人脑组织的抗自由基活性。
A superoxide dismutase ( SOD) biosensor for determination of superoxide radicals has been developed by immobilization of superoxide dismutase within gelatin ( G) on a Pt electrode surface. The properties of the biosensor have been investigated and optimum conditions-enzyme concentration, glutaraldehyde concentration, and pH-were determined. The response of the G-SOD biosensor was proportional to O-2-(center dot-) concentration and the detection limit was 0.01 mmol L-1 at a signal-to-noise ratio of 3. The biosensor retained 89% and 60% of its sensitivity after use for three and four weeks, respectively. Immobilization of SOD on gelatin provides a biocompatible microenvironment around the enzyme and stabilizes the activity of the enzyme very efficiently. The superoxide dismutase biosensor was used to determine the antioxidant properties of acetylsalicylic acid-based drugs and the anti-radical activity of healthy and cancerous human brain tissues.