Macular pigment density measured by autofluorescence spectrometry: comparison with reflectometry and heterochromatic flicker photometry
Macular pigment density measured by autofluorescence spectrometry: comparison with reflectometry and heterochromatic flicker photometry
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DOI：
10.1364/josaa.18.001212
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发表时间：
2001-06-01
影响因子：
1.9
通讯作者：
Burns, SA
中科院分区：
文献类型：
--
作者：
Delori, FC;Goger, DG;Burns, SA
我们提出了一种技术来估计人类黄斑色素的密度无创，利用脂褐素的自身荧光，这是通常存在于人类视网膜色素上皮。通过测量黄斑色素基本为零吸收的710 nm处的荧光强度，并用两个波长刺激荧光，一个波长被黄斑色素吸收良好，另一个波长被黄斑色素吸收最低，我们可以一次准确地测量黄斑色素密度。我们使用该技术测量了159名年龄在15至80岁之间视网膜状态正常的受试者的黄斑色素密度。在2度直径的试验场中，平均黄斑色素密度为0.48±0.16密度单位（D.U.）。我们发现，这些估计值与同一受试者中黄斑色素的反射估计值（平均值：0.23 +/- 0.07 D.U.）和心理物理估计值（平均值：0.37 +/- 0.26 D.U.；通过异色闪烁光度法获得）高度相关，尽管不同技术之间的估计密度存在系统性差异。在短时间间隔和长时间间隔内重复测量表明，自体荧光技术具有可重复性：估计之间的平均绝对差小于0.05 D.U，优于反射法和闪烁光度法获得的重复性。为了理解从不同方法获得的密度估计值之间的系统差异，我们分析了每种技术的基本假设。具体来说，我们研究了视觉色素的自我筛选作用，视网膜深层光学特性变化的影响，包括视网膜色素上皮黑色素的作用，以及视网膜前层的次级荧光团和反射器的作用。(C) 2001美国光学学会。
We present a technique for estimating the density of the human macular pigment noninvasively that takes advantage of the autofluorescence of lipofuscin, which is normally present in the human retinal pigment epithelium. By measuring the intensity of fluorescence at 710 nm, where macular pigment has essentially zero absorption, and stimulating the fluorescence with two wavelengths, one well absorbed by macular pigment and the other minimally absorbed by macular pigment, we can make accurate single-pass measurements of the macular pigment density. We used the technique to measure macular pigment density in a group of 159 subjects with normal retinal status ranging in age between 15 and 80 years. Average macular pigment density was 0.48 +/- 0.16 density unit (D.U.) for a 2 degrees -diameter test field. We show that these estimates are highly correlated with reflectometric (mean: 0.23 +/- 0.07 D.U.) and psychophysical (mean: 0.37 +/- 0.26 D.U.; obtained by heterochromatic flicker photometry) estimates of macular pigment in the same subjects, despite the fact that systematic differences in the estimated density exist between techniques. Repeat measurements over both short- and long-time intervals indicate that the autofluorescence technique is reproducible: The mean absolute difference between estimates was less than 0.05 D.U., superior to the reproducibility obtained by reflectometry and flicker photometry. To understand the systematic differences between density estimates obtained from the different methods, we analyzed the underlying assumptions of each technique. Specifically, we looked at the effect of self-screening by visual pigment, the effect of changes in optical property of the deeper retinal layers, including the role of retinal pigmented epithelium melanin, and the role of secondary fluorophores and reflectors in the anterior layers of the retina. (C) 2001 Optical Society of America.