The DrosDel deletion collection:: A Drosophila genomewide chromosomal deficiency resource
The DrosDel deletion collection:: A Drosophila genomewide chromosomal deficiency resource
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DOI：
10.1534/genetics.107.076216
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发表时间：
2007-09-01
期刊：
影响因子：
3.3
通讯作者：
Russell, Steven
中科院分区：
文献类型：
--
作者：
Ryder, Edward;Ashburner, Michael;Russell, Steven
我们描述了一个第二基因定量缺乏试剂盒果蝇组成的分子定位缺失的同基因背景，覆盖类似的77%的版本5.1基因组。使用先前报道的收集FRT轴承P-元件插入，我们已经产生了655个新的删除和验证了一组209删除轴承苍蝇股票。除了缺失，我们还展示了P元件如何也可用于生成一组自定义倒位和重复，特别适用于平衡携带单倍不足位点的基因组的困难区域。我们描述了一个简单的计算资源，便于选择适当的元素生成自定义删除。最后，我们提供了一个计算资源，便于选择其他映射的FRT轴承元素，当与DrosDel集合相结合，理论上可以产生超过50万个精确映射的删除。
We describe a second-gene ration deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering similar to 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.