Wounding of Arabidopsis leaves causes a powerful but transient protection against Botrytis infection
Wounding of Arabidopsis leaves causes a powerful but transient protection against Botrytis infection
复制标题
DOI：
10.1111/j.1365-313x.2008.03540.x
复制
发表时间：
2008-08-01
期刊：
影响因子：
7.2
通讯作者：
Lamotte, Olivier
中科院分区：
文献类型：
--
作者：
Chassot, Celine;Buchala, Antony;Lamotte, Olivier
对活组织造成的物理损伤使其容易受到病原体的侵袭。然而，拟南芥叶片的损伤不符合这一概念，并导致对灰霉病菌（Botrytis cinerea）的免疫。在受伤的叶片，菌丝生长受到强烈抑制相比，未受伤的控制。伤口诱导的电阻与水杨酸，茉莉酸或乙烯依赖的防御反应。发现植物抗毒素camalexin参与这种防御反应，因为camalexin缺陷突变体在受伤后不受保护，并且B。这里使用的灰霉病菌菌株对该化合物敏感。单独的创伤不会导致Camalexin的产生，但接种B后引发其积累。灰霉病，进一步支持了camalexin在伤口诱导的抗性中的作用。与增加的Camalexin生产平行，与未受伤和感染的植物相比，在受伤和感染的植物中更快地诱导参与Camalexin生物合成的基因。还发现谷氨酸是抗性所需的，因为γ-谷氨酰半胱氨酸合成酶缺陷的突变体显示对B的敏感性。灰霉病受伤后，表明野生型谷胱甘肽的基础水平所需的创伤诱导的电阻。此外，编码谷胱甘肽-S-转移酶1的基因的表达通过接种B的叶片中的创伤引发。灰绿色此外，还观察到B对受伤叶片MAP激酶活性的激发作用。与未受伤的接种对照相比，灰霉病。我们的研究结果证明了非生物胁迫如何诱导对B强毒株的免疫。灰霉病，一个涉及camalexin和谷胱甘肽的过程。
Physical injury inflicted on living tissue makes it vulnerable to invasion by pathogens. Wounding of Arabidopsis thaliana leaves, however, does not conform to this concept and leads to immunity to Botrytis cinerea, the causal agent of grey mould. In wounded leaves, hyphal growth was strongly inhibited compared to unwounded controls. Wound-induced resistance was not associated with salicylic acid-, jasmonic acid- or ethylene-dependent defence responses. The phytoalexin camalexin was found to be involved in this defence response as camalexin-deficient mutants were not protected after wounding and the B. cinerea strains used here were sensitive to this compound. Wounding alone did not lead to camalexin production but primed its accumulation after inoculation with B. cinerea, further supporting the role of camalexin in wound-induced resistance. In parallel with increased camalexin production, genes involved in the biosynthesis of camalexin were induced faster in wounded and infected plants in comparison with unwounded and infected plants. Glutathione was also found to be required for resistance, as mutants deficient in gamma-glutamylcysteine synthetase showed susceptibility to B. cinerea after wounding, indicating that wild-type basal levels of glutathione are required for the wound-induced resistance. Furthermore, expression of the gene encoding glutathione-S-transferase 1 was primed by wounding in leaves inoculated with B. cinerea. In addition, the priming of MAP kinase activity was observed after inoculation of wounded leaves with B. cinerea compared to unwounded inoculated controls. Our results demonstrate how abiotic stress can induce immunity to virulent strains of B. cinerea, a process that involves camalexin and glutathione.