Inhibitory neurons in the human epileptogenic temporal neocortex - An immunocytochemical study
Inhibitory neurons in the human epileptogenic temporal neocortex - An immunocytochemical study
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DOI：
10.1093/brain/119.4.1327
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发表时间：
1996-08-01
期刊：
影响因子：
14.5
通讯作者：
DeFelipe, J
中科院分区：
文献类型：
--
作者：
Marco, P;Sola, RG;DeFelipe, J
采用免疫细胞化学方法研究在手术治疗与脑肿瘤相关或无关的顽固性颞叶癫痫期间切除的人新皮质中抑制性神经元回路的变化。通过术中皮质电图（ECOG）对致癫痫皮层进行表征并分为尖峰区或非尖峰区。将切除的皮质切成块，切片并进行免疫细胞化学染色，以观察谷氨酸脱羧酶（GAD）、钙结合蛋白、小白蛋白（PV）和胶质纤维酸性蛋白（GFAP）。通过免疫细胞化学和组织学方法发现了皮质神经元回路的多种改变。抑制性神经元回路中的类似改变似乎独立于主要致癫痫部位和与癫痫相关的病理学而发生，这表明可能存在导致癫痫发作活动的共同基本机制。这些变化显然与手术时的ECOG发现无关，这使人们对使用ECOG记录的发作间期癫痫放电来指导皮质切除的价值产生了疑问。最显着和最常见的变化是吊灯单元的丢失。这些细胞是致癫痫颞叶皮质中最脆弱的 GABA 能中间神经元类型，这一发现表明它们可能具有重要的功能，因为吊灯细胞的轴突末端可能对皮质锥体细胞的冲动产生发挥强大的调节作用。因此，这些细胞可能代表人类癫痫病因学的关键组成部分。
Immunocytochemical methods were used to study alterations in inhibitory neuronal circuits in human neocortex resected during surgical treatment of intractable temporal epilepsy associated or not with brain tumours. The epileptogenic cortex was characterized and divided into spiking or non-spiking zones by intraoperative electrocorticography (ECOG). The resected cortex was cut into blocks, sectioned and stained immunocytochemically for visualization of glutamic acid decarboxylase (GAD), the calcium-binding protein, parvalbumin (PV) and glial fibrillary acidic protein (GFAP). A variety of alterations in cortical neuronal circuits as revealed by immunocytochemical and histological methods were found. Similar alterations in inhibitory neuronal circuits appear to occur independently of the primary epileptogenic site and pathology associated with epilepsy which suggests that there is possibly a common basic underlying mechanism that lends to seizure activity These changes were apparently unrelated to ECOG findings at surgery, which bring into question the value of the use of interictal epileptic discharges recorded by ECOG to guide cortical resections. The most conspicuous and common change was the loss of chandelier cells. The finding that these cells are among the most vulnerable types of GABAergic interneurons in the epileptogenic temporal cortex indicates that they might be of great functional importance, since the axon terminals of chandelier cells ape likely to exert powerful regulation of impulse generation in cortical pyramidal cells. Therefore, these cells might represent a key component in the aetiology of human epilepsy.