Study of the interaction between rabbit cardiac contractile and regulatory proteins. An in vitro motility assay
Study of the interaction between rabbit cardiac contractile and regulatory proteins. An in vitro motility assay
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兔心肌收缩与调节蛋白相互作用的研究。
DOI：
10.1134/s0006297908020090
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
L. Katsnelson
中科院分区：
文献类型：
--
作者：
L. Nikitina;G. Kopylova;D. Shchepkin;L. Katsnelson
一系列的实验进行了重建细丝在体外运动试验，以获得pCa力的关系，心脏异肌球蛋白V1和V3。检测了流动池表面上两种浓度的每种异肌球蛋白（200和300 μg/ml）。等距力估计为肌动蛋白结合蛋白α-辅肌动蛋白的量，阻止细丝运动。结果发现，在两种异构体浓度下，V3在饱和钙浓度下停止运动的α-辅肌动蛋白的量是V1的两倍。确定了pCa-力关系的希尔协同系数（h）。在300 μg/ml蛋白质浓度下，同种型的h值无显著差异（V1和V3的h分别为1.56和1.54）。然而，200 μg/ml时V3亚型的希尔系数较高（V3和V1的h分别为2.00和1.76）。重要的是，希尔系数增加两种同工酶时，其浓度降低。详细分析了Hill系数与心肌收缩蛋白和调节蛋白之间协同作用的关系。
A series of experiments was performed in an in vitro motility assay with reconstructed thin filaments to obtain pCa-force relationships for cardiac isomyosins V1 and V3. Two concentrations of each isomyosin (200 and 300 μg/ml) on the surface of a flow cell were tested. Isometric force was estimated as the amount of actin-binding protein, α-actinin, stopping thin filament movement. It was found that the amount of α-actinin stopping the movement at saturating calcium concentration for V3 was twice higher than for V1 at both concentrations of isoforms. Hill coefficients of cooperativity (h) were determined for pCa-force relationships. The value of h did not differ significantly for isoforms at 300 μg/ml of protein (h was 1.56 for V1 and 1.54 for V3). However, the Hill coefficient was higher for V3 isoform at 200 μg/ml (h = 2.00 and 1.76 for V3 and V1, respectively). Importantly, the Hill coefficient increased for both isoenzymes when their concentrations were decreased. The connection between Hill coefficient and cooperative interactions between cardiac contractile and regulatory proteins is analyzed in detail.
DOI：
10.1016/s0021-9258(17)43827-0
发表时间：
1983-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Z. Grabarek;J. Grabarek;Paul C. LeavisS;J. Gergely
通讯作者：
Z. Grabarek;J. Grabarek;Paul C. LeavisS;J. Gergely