Are Variable-Number Tandem Repeats Appropriate for Genotyping Mycobacterium leprae?
Are Variable-Number Tandem Repeats Appropriate for Genotyping Mycobacterium leprae?
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DOI：
10.1128/jcm.00239-08
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发表时间：
2008-05
影响因子：
9.4
通讯作者：
M. Monot;N. Honoré;C. Balière;B. Ji;S. Sow;P. Brennan;S. Cole
中科院分区：
文献类型：
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作者：
M. Monot;N. Honoré;C. Balière;B. Ji;S. Sow;P. Brennan;S. Cole
摘要对泰米尔纳德邦麻风分枝杆菌菌株的比较基因组学分析发现了几个多态性位点，这些位点有可能作为流行病学工具。本研究比较了两种不同的M.在从同一麻风病人分离的几个活检样本中发现麻风菌。第一种类型包括五种不同的可变数目串联重复序列（VNTR），而第二种类型由三种单核苷酸多态性（SNP）组成。获得了对比结果，因为在M的SNP谱中没有观察到变化。来自马里7个不同地点的42名麻风患者的VNTR图谱差异很大。此外，由于VNTR图谱的变化不仅在不同的M.但是，由于VNTR在麻风病的流行病学标志物中的动态性，而且在来自同一患者的活检样品之间的动态性，这些VNTR可能太动态而不能用作麻风病的流行病学标志物。
ABSTRACT Comparative genomics analysis of the Tamil Nadu strain of Mycobacterium leprae has uncovered several polymorphic sites with potential as epidemiological tools. In this study we compared the stability of two different markers of genomic biodiversity of M. leprae in several biopsy samples isolated from the same leprosy patient. The first type comprises five different variable-number tandem repeats (VNTR), while the second is composed of three single nucleotide polymorphisms (SNP). Contrasting results were obtained, since no variation was seen in the SNP profiles of M. leprae from 42 patients from 7 different locations in Mali whereas the VNTR profiles varied considerably. Furthermore, since variation in the VNTR pattern was seen not only between different isolates of M. leprae but also between biopsy samples from the same patient, these VNTR may be too dynamic for use as epidemiological markers for leprosy.