Suppression of apolipoprotein M expression and secretion in alloxan-diabetic mouse:: Partial reversal by insulin
Suppression of apolipoprotein M expression and secretion in alloxan-diabetic mouse:: Partial reversal by insulin
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DOI：
10.1016/j.bbrc.2006.02.022
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发表时间：
2006-04-21
影响因子：
3.1
通讯作者：
Ahrén, B
中科院分区：
文献类型：
--
作者：
Xu, N;Nilsson-Ehle, P;Ahrén, B
我们以前报道过载脂蛋白M（apoM）的表达在ob/ob小鼠中减少。这种减少是否是该模型特有的，还是糖尿病模型中更普遍的现象尚不清楚。因此，在本研究中，我们研究了四氧嘧啶（120 mg/kg）给药诱发糖尿病的NMR 1小鼠apoM的表达和分泌。四氧嘧啶后第3天血糖水平显著升高，血浆胰岛素水平显著降低。与此同时，与注射生理盐水的对照组相比，四氧嘧啶处理的小鼠血浆apoM浓度降低70%，肝脏apoM mRNA水平降低40%，肾脏apoM mRNA水平降低20%。此外，我们还发现每天皮下注射胰岛素（51 U/kg/d）可增加血浆apoM水平，并增加肝脏和肾脏apoMmRNA水平。因此，我们得出结论，载脂蛋白M是减少在这个糖尿病模型和外源性胰岛素管理部分逆转异常载脂蛋白M的表达。基于这些结果，我们认为，胰岛素调节apoM的合成在体内，因此，apoM的表达减少是一种普遍现象，在糖尿病模型。(c)2006爱思唯尔公司All rights reserved.
We previously reported that apolipoprotein M (apoM) expression is reduced in ob/ob mice. Whether such a reduction is specific for this model or is a more general phenomenon in diabetes models is not known. In the present study, we therefore investigated apoM expression and secretion in NMR1 mice tendered diabetes through administration of alloxan (120 mg/kg). Plasma glucose levels were markedly increased and plasma insulin levels markedly reduced at 3 days after alloxan. At the same time, that plasma apoM concentrations were decreased by 70%, apoM mRNA levels in liver was decreased by 40%, and apoM mRNA in kidney was decreased by 20% in alloxan-treated mice compared to saline-injected controls. Furthermore we found also that daily sc administration of insulin (51U/kg per day) increased plasma apoM levels, and apoM mRNA levels in liver and kidney. We therefore conclude that apoM is reduced in this diabetes model and that exogenous insulin administrations partially reverses the abnormal apoM expression. Based on these results, we suggest that insulin regulates apoM synthesis in vivo and, therefore, that the reduction of apoM expression is a general phenomenon in diabetes models. (c) 2006 Elsevier Inc. All rights reserved.