Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway
Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway
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DOI：
10.1073/pnas.231442498
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发表时间：
2001-10-23
影响因子：
11.1
通讯作者：
Norgard, MV
中科院分区：
文献类型：
--
作者：
Hübner, A;Yang, XF;Norgard, MV
RpoS和RpoN是两个典型地与细菌中的一般应激反应相关的替代西格玛因子。到目前为止，还没有实验证据表明RpoS和RpoN可以直接控制彼此的表达。在此，使用基因破坏和靶向伯氏疏螺旋体菌株297中的rpoN和rpoS的遗传互补的组合策略，我们描述了B的调控网络。burgdorferi。在该网络中，RpoN控制RpoS的表达，RpoS又控制两种重要的膜脂蛋白的表达，即外表面蛋白C和核心蛋白聚糖结合蛋白A，以及可能的B的其他蛋白。burgdorferi。我们的研究结果为进一步阐明可能影响B许多方面的关键调控网络提供了基础。burgdorferi的寄生策略，宿主范围和毒力表达。
RpoS and RpoN are two alternative sigma factors typically associated with general stress responses in bacteria. To date, there has been no experimental evidence that RpoS and RpoN can directly control the expression of one another. Herein, using a combined strategy of gene disruption and genetic complementation targeting rpoN and rpoS in Borrelia burgdorferi strain 297, we describe a regulatory network for B. burgdorferi. In this network, RpoN controls the expression of RpoS, which, in turn, governs the expression of two important membrane lipoproteins, outer surface protein C and decorin-binding protein A, and likely other proteins of B. burgdorferi. Our findings provide a foundation for elucidating further key regulatory networks that potentially impact many aspects of B. burgdorferi's parasitic strategy, host range, and virulence expression.