Poly(ethylene glycol)-functionalized polymeric microchips for capillary electrophoresis.
Poly(ethylene glycol)-functionalized polymeric microchips for capillary electrophoresis.
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DOI：
10.1021/ac9001832
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发表时间：
2009-07
影响因子：
7.4
通讯作者：
Xuefei Sun;Dan-dan Li;Milton L. Lee
中科院分区：
文献类型：
--
作者：
Xuefei Sun;Dan-dan Li;Milton L. Lee
Recently, we reported the synthesis, fabrication, and preliminary evaluation of poly(ethylene glycol) (PEG)-functionalized polymeric microchips that are inherently resistant to protein adsorption without surface modification in capillary electrophoresis (CE). In this study, we investigated the impact of cross-linker purity and addition of methyl methacrylate (MMA) as a comonomer on CE performance. Impure poly(ethylene glycol) diacrylate (PEGDA) induced electroosmotic flow (EOF) and increased the separation time, while the addition of MMA decreased the separation efficiency to approximately 25% of that obtained using microchips fabricated without MMA. Resultant improved microchips were evaluated for the separation of fluorescent dyes, amino acids, peptides, and proteins. A CE efficiency of 4.2 x 10(4) plates for aspartic acid in a 3.5 cm long microchannel was obtained. Chiral separation of 10 different D,L-amino acid pairs was obtained with addition of a chiral selector (i.e., beta-cyclodextrin) in the running buffer. Selectivity (alpha) and resolution (R(s)) for D,L-leucine were 1.16 and 1.64, respectively. Good reproducibility was an added advantage of these PEG-functionalized microchips.
