Replication of ocular isolates of human adenovirus is serotype-dependent in rabbit corneal organ culture.
Replication of ocular isolates of human adenovirus is serotype-dependent in rabbit corneal organ culture.
复制标题
人腺病毒眼部分离株在兔角膜器官培养中的复制具有血清型依赖性。
作者：
Y. Gordon;T. Araullo;E. Romanowski;B. Myers;D. Santora;M. Lin;R. Kowalski
本体外研究的目的是确定未适应的人腺病毒眼部分离株在兔角膜中复制的能力。在器官培养物（24 孔板）中生长的兔角膜局部接种 50 微升（5 x 10(5) pfu）不同眼部腺病毒血清型（ATCC 和临床分离株）。对照孔（不存在角膜）以类似的方式接种。通过对 A549 细胞进行连续等分滴定来确定病毒复制。我们证明了所测试的 Ad1、2、5、6、8、9、11 和 37 的所有分离株（100%）随时间的持续病毒复制。 Ad3、7A、19 和 4 的任何分离株 (0%) 均未在我们的模型中表现出复制性。成功血清型之间以及给定血清型的不同分离株之间的峰值滴度从 10(2) pfu/ml (Ad11) 到 10(5) PFU/ml (Ad5) 不等。我们得出的结论是，未适应的人类 Ad 血清型在兔角膜中复制的能力具有血清型依赖性，并且 C 亚组（Ad1、2、5 和 6）似乎是最成功的亚组。
The goal of the present in vitro study was to determine the ability of unadapted human adenoviral ocular isolates to replicate in the rabbit cornea. Rabbit corneas grown in organ culture (24 well plate) were inoculated topically with 50 microliters (5 x 10(5) pfu) of different ocular adenoviral serotypes (ATCC and clinical isolates). Control wells (no cornea present) were inoculated in a similar fashion. Viral replication was determined by serial aliquots titrated on A549 cells. We demonstrated sustained viral replication over time of all isolates (100%) of Ad1, 2, 5, 6, 8, 9, 11 and 37 tested. No isolates (0%) of Ad3, 7A, 19, and 4 demonstrated replication in our model. Peak titers varied among successful serotypes from 10(2) pfu/ml (Ad11) to 10(5) PFU/ml (Ad5), and among different isolates of a given serotype. We conclude that the ability of unadapted human Ad serotypes to replicate in rabbit corneas was serotype-dependent, and that subgroup C (Ad1, 2, 5, and 6) appeared to be the most successful subgroup.