Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds
Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds
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DOI：
10.1046/j.1365-313x.2000.00663.x
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发表时间：
2000-01-01
期刊：
影响因子：
7.2
通讯作者：
Holdsworth, MJ
中科院分区：
文献类型：
--
作者：
Kurup, S;Jones, HD;Holdsworth, MJ
ABI3 基因座是拟南芥胚胎发育的主要调节因子，对于同时激活成熟途径以及抑制发芽和幼苗发育至关重要。我们在酵母中使用了双杂交筛选来鉴定与 ABI3 相互作用的蛋白质。鉴定出四种 ABI3 相互作用蛋白 (AIP)，它们在体内和体外表现出与包含 B2 和 B3 结构域的 ABI3 C 末端区域的特异性相互作用，先前显示该区域具有 DNA 结合活性。编码 AIP 的基因的表达特征也在野生型和 abi3、lec1 和 fus3 胚胎突变体中进行了分析。该分析证明了这些基因在正常胚胎发育过程中和突变系中的差异表达。所有 AIP 均与现有转录因子具有同源性，因此它们可能与控制拟南芥胚胎发育的转录调节因子网络中的 ABI3 一起发挥作用。
The ABI3 locus is a major regulator of embryo development in Arabidopsis and is essential for the simultaneous activation of the maturation pathway, as well as repression of germination and seedling development. We used a two-hybrid screen in yeast in order to identify proteins that interact with ABI3. Four ABI3-interacting proteins (AIPs) were identified which showed specific in vivo and in vitro interactions with the C-terminal region of ABI3 that contains the B2 and B3 domains, previously shown to have DNA binding activity. The expression characteristics of the genes encoding the AIPs have also been analysed in wild-type and abi3, lec1 and fus3 embryo mutants. This analysis demonstrated differential expression of these genes during normal embryo development and in the mutant lines. All the AIPs show homology to existing transcription factors and therefore they may function with ABI3 within the network of transcriptional regulators that control embryo development in Arabidopsis.