THE GENE SERRATE ENCODES A PUTATIVE EGF-LIKE TRANSMEMBRANE PROTEIN ESSENTIAL FOR PROPER ECTODERMAL DEVELOPMENT IN DROSOPHILA-MELANOGASTER
THE GENE SERRATE ENCODES A PUTATIVE EGF-LIKE TRANSMEMBRANE PROTEIN ESSENTIAL FOR PROPER ECTODERMAL DEVELOPMENT IN DROSOPHILA-MELANOGASTER
复制标题
DOI：
10.1101/gad.4.12a.2188
复制
发表时间：
1990-12-01
影响因子：
10.5
通讯作者：
ARTAVANISTSAKONAS, S
中科院分区：
文献类型：
--
作者：
FLEMING, RJ;SCOTTGALE, TN;ARTAVANISTSAKONAS, S
第三染色体基因Serrate中的突变显示出与神经原性基因座Notch的特定等位基因的遗传相互作用，Notch编码具有表皮生长因子（EGF）同源性的跨膜蛋白。胚胎致死性锯齿突变表现出表皮和神经元缺陷，这让人想起由果蝇EGF受体同源基因突变产生的缺陷。我们克隆了Serrate的分子克隆，发现它编码两个长度大于30 kb的基因组片段的协同表达的转录本。该蛋白由1404个氨基酸组成，含有一个跨膜结构域和14个EGF样重复序列。因此，Serrate代表果蝇中含EGF基因座组的另一个成员。整体安装原位杂交分析揭示了复杂的时间和空间模式的RNA表达与突变胚胎中观察到的表皮和神经元缺陷相一致。最后，我们讨论了已知参与外胚层衍生组织分化的其他细胞表面分子的背景下的锯齿功能的影响。
Mutation in the third chromosome gene Serrate are shown to display genetic interactions with specific alleles of the neurogenic locus Notch, which encodes a transmembrane protein with epidermal growth factor (EGF) homology. Embryonic lethal Serrate mutations exhibit epidermal and neuronal defects, which are reminiscent of those produced by mutations in the Drosohila EGF receptor homolog gene. We present the molecular clong of Serrate and show that it encodes two coordinately expressed transcripts from a genomic interval > 30 kb in length. The deduced protein product of 1404 amino acids contains a single transmembrane domain and 14 EGF-like repeats. Thus, Serrate represents another member of the group of EGF-containing loci in Drosophila. Whole-mount in situ hybridization analysis reveals complex temporal and spatial patterns of RNA expression consistent with the epidermal and neuronal defects observed in mutant embryos. Finally, we discuss the implications of Serrate function within the context of other cell-surfce molecules known to be involved in the differentiation of ectodermally derived tissues.