Bloom helicase and DNA topoisomerase IIIα are involved in the dissolution of sister chromatids
Bloom helicase and DNA topoisomerase IIIα are involved in the dissolution of sister chromatids
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DOI：
10.1128/mcb.00702-06
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发表时间：
2006-08-01
影响因子：
5.3
通讯作者：
Enomoto, Takemi
中科院分区：
文献类型：
--
作者：
Seki, Masayuki;Nakagawa, Takayuki;Enomoto, Takemi
布卢姆综合征（Bloom's syndrome，BS）是一种常染色体疾病，其特征是易患多种癌症.其突变导致BS的基因产物是RecQ家族解旋酶BLM，其与DNA拓扑异构酶III β（Top3 α）形成复合物。然而，细胞内BLM和Top3 α之间相互作用的生理相关性仍不清楚。我们在这里表明，前3 α耗竭导致积累的细胞在G，期，扩大的细胞核，和染色体的差距和断裂发生在同一位置的姐妹染色单体。从中期到后期的转变也受到抑制。所有这些现象，除了细胞致死性抑制BLM基因破坏。结合BLM和Top3 α的生物化学性质，这些数据表明BLM和Top3 α执行姐妹染色单体的溶解。
Bloom's syndrome (BS) is an autosomal disorder characterized by predisposition to a wide variety of cancers. The gene product whose mutation leads to BS is the RecQ family helicase BLM, which forms a complex with DNA topoisomerase III beta (Top3 alpha). However, the physiological relevance of the interaction between BLM and Top3 alpha within the cell remains unclear. We show here that Top3 alpha depletion causes accumulation of cells in G, phase, enlargement of nuclei, and chromosome gaps and breaks that occur at the same position in sister chromatids. The transition from metaphase to anaphase is also inhibited. All of these phenomena except cell lethality are suppressed by BLM gene disruption. Taken together with the biochemical properties of BLM and Top3 alpha, these data indicate that BLM and Top3 alpha execute the dissolution of sister chromatids.