Humoral and cell mediated immune responses to a pertussis containing vaccine in pregnant and nonpregnant women
Humoral and cell mediated immune responses to a pertussis containing vaccine in pregnant and nonpregnant women
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DOI：
10.1016/j.vaccine.2015.06.108
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发表时间：
2015-08-07
期刊：
影响因子：
5.5
通讯作者：
Leuridan, Elke
中科院分区：
文献类型：
--
作者：
Huygen, Kris;Cabore, Raissa Nadege;Leuridan, Elke
对于某些传染病，建议孕妇接种疫苗，以便通过母体IgG抗体的高滴度保护妇女和后代。关于怀孕期间接种疫苗引发细胞免疫反应的情况知之甚少。在一项正在进行的母体百日咳疫苗接种研究（2012 - 2014年）中，18名孕妇在妊娠晚期接种了含破伤风-白喉-无细胞百日咳（Tdap）的疫苗（Boostrix（R））。16名年龄匹配的非孕妇在同一时间段接种了相同的疫苗。在接种疫苗时、接种疫苗前以及接种疫苗后1个月和1年采集血样。ELISA法检测百日咳毒素（PT）、丝状血凝素（FHA）、百日咳毒素（Pm）、破伤风毒素（TT）和白喉毒素（DT）抗体。细胞免疫反应进行了分析，使用稀释的全血测定，测量增殖，和细胞因子释放的疫苗抗原PT，FHA，TT，和美洲商陆有丝分裂原（PWM）作为多克隆stimulation.Antibody水平的所有五个疫苗成分的增加显着，并在相同程度上接种后，在怀孕和非怀孕的妇女。接种疫苗后1年，抗体滴度下降，尤其是PT，但所有抗原的抗体滴度仍显著高于接种前。相比之下，非妊娠女性在接种疫苗后1个月对TT、PT和FHA的增殖和IFN-γ应答增加，而妊娠女性中仅TT特异性T细胞应答增加，且程度低于对照组。接种疫苗后一年，两组的细胞应答与接种疫苗前检测到的基线水平相同。总之，Tdap疫苗接种可以增加疫苗特异性IgG抗体在怀孕和非怀孕妇女在相同程度上，而刺激疫苗特异性Th1型细胞免疫应答与这种无细胞疫苗是短暂的，在怀孕期间受损。(C)2015爱思唯尔有限公司版权所有。
Vaccination of pregnant women is recommended for some infectious diseases in order to protect both women and offspring through high titres of maternal IgG antibodies. Less is known on the triggering of cellular immune responses by vaccines administered during pregnancy. In an ongoing study on maternal pertussis vaccination (2012-2014) 18 pregnant women were vaccinated with a tetanus-diphtheria-acellular pertussis (Tdap) containing vaccine (Boostrix (R)) during the third pregnancy trimester. Sixteen age-matched nonpregnant women received the same vaccine in the same time period. A blood sample was taken at the moment of, but before vaccination and one month and one year after vaccination. Anti-Pertussis Toxin (PT), filamentous hemagglutinin (FHA), pertactin (Pm), tetanus toxin (TT) and diphtheria toxin (DT) antibodies were measured by ELISA. Cellular immune responses were analyzed using a diluted whole blood assay, measuring proliferation, and cytokine release in response to vaccine antigens PT, FHA, TT, and to pokeweed mitogen (PWM) as polyclonal stimulus.Antibody levels to all five vaccine components increased significantly and to the same extent after vaccination in pregnant and nonpregnant women. One year after vaccination, antibody titres had decreased particularly to PT, but they were still significantly higher to all antigens than before vaccination. In contrast, proliferative and IFN-gamma responses were increased to TT, PT, and FHA in nonpregnant women one month after vaccination, whereas in pregnant women only TT specific T cell responses were increased and to a lesser extent than in the control group. One year after vaccination, cellular responses equaled the baseline levels detected prior to vaccination in both groups. In conclusion, a Tdap vaccination can increase vaccine specific IgG antibodies to the same extent in pregnant and in nonpregnant women, whereas the stimulation of vaccine specific Th1 type cellular immune responses with this acellular vaccine is transient and impaired during pregnancy. (C) 2015 Elsevier Ltd. All rights reserved.