Preparation of NIR-responsive, ROS-generating and antibacterial black phosphorus quantum dots for promoting the MRSA-infected wound healing in diabetic rats
Preparation of NIR-responsive, ROS-generating and antibacterial black phosphorus quantum dots for promoting the MRSA-infected wound healing in diabetic rats
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DOI：
10.1016/j.actbio.2021.10.008
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发表时间：
2021-12-11
影响因子：
9.7
通讯作者：
Mei, Xifan
中科院分区：
文献类型：
--
作者：
Huang, Shuocheng;Xu, Shibo;Mei, Xifan
耐多药（MDR）细菌引起的感染正在成为临床治疗的一个巨大挑战，特别是对于不愈合的糖尿病伤口感染，它增加了患者的死亡率。MRSA感染和伤口愈合延迟（耐甲氧西林金黄色葡萄球菌）占较高比例。虽然手术清创和持续使用抗生素仍然是主要的临床治疗方法，但新的多功能治疗纳米平台对MIDW具有吸引力。因此，本研究利用水凝胶（BPQDs@NH）封装的黑磷量子点（BPQDs）作为MIDW处理的纳米平台，实现了近红外（NIR）响应性、活性氧（ROS）生成和抗菌活性的多功能特性。在近红外照射下，BPQDs@NH-treated MIDW区域的温度迅速升高到55摄氏度，用于灭菌。体外实验表明，BPQDs@NH在近红外照射下通过ROS的产生、脂质过氧化、谷胱甘肽、三磷酸腺苷的积累和细菌膜的破坏，发挥协同抑制MRSA的作用。由此产生的BPQDs@NH对MRSA的有效灭菌率接近90%。此外，动物实验显示BPQDs@NH通过降低炎症反应和调节血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的表达，使12天后MIDW的有效闭合率达到95%。静脉循环实验表明，BPQDs具有良好的生物相容性，对大鼠主要脏器无明显损伤。结果表明，BPQDs@NH实现了nir反应性、ROS生成和抗菌活性的协同作用，促进了伤口愈合，表明它们是很有前景的MIDW愈合多功能纳米平台。意义陈述nir触发的ros生成和抗菌纳米平台在伤口愈合领域具有广泛的应用前景。2. 在这项工作中，包裹在水凝胶中的黑磷量子点被用作治疗MRSA感染伤口的纳米平台。3. 获得的材料达到了MRSA的有效灭菌率和有效的伤口闭合率。(C) 2021材料学报Elsevier Ltd.出版。版权所有。
Multidrug-resistant (MDR) bacteria-induced infection is becoming a huge challenge for clinical treat-ment, especially for non-healing diabetic wound infections, which increase patient mortality. MRSA in-fections and delayed wound healing (methicillin-resistant Staphylococcus aureus) accounted for a higher proportion. Although surgical debridement and continuous use of antibiotics are still the main clinical treatments, new multifunctional therapeutic nanoplatform are attractive for MIDW. Thus, in the present study, black phosphorus quantum dots (BPQDs) encapsulated in hydrogel (BPQDs@NH) were utilized as nanoplatforms for MIDW treatment to achieve the multifunctional properties of NIR (near infrared) re-sponsiveness, ROS (reactive oxygen species) generation and antibacterial activity. Upon NIR irradiation, the temperature of the BPQDs@NH-treated MIDW area rapidly increased up to 55 degrees C for sterilization. In vitro experiments showed that BPQDs@NH exerted a synergistic effect on inhibiting MRSA by produc-ing of ROS, lipid peroxidation, glutathione, adenosine triphosphate accumulation and bacterial membrane destruction upon NIR irradiation. The resulting BPQDs@NH achieved an effective sterilization rate of ap-proximately 90% for MRSA. Furthermore, animal experiments revealed that BPQDs@NH achieved an ef-fective closure rate of 95% for MIDW after 12 days by reducing the inflammatory response and regulating the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). Meanwhile, intravenous circulation experiments showed good biocompatibility of BPQDs, and no obvi-ous damage to rat major organs was observed. The obtained results indicated that BPQDs@NH achieved the synergistic functions of NIR-responsiveness, ROS generation, and antibacterial activity and promoted wound healing, suggesting that they are promising multifunctional nanoplatforms for MIDW healing.Statement of significance1. NIR-triggered ROS-generating and antibacterial nanoplatforms are attractive in the wound healing field. 2. In this work, black phosphorus quantum dots encapsulated in a hydrogel were used as a nanoplatform for treating MRSA infected wounds. 3. The obtained materials have achieved an effective sterilization rate for MRSA and effective wound closure rate.(C) 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.