Mass rearing of temperature sensitive genetic sexing strains in the Mediterranean fruit fly (Ceratitis capitata)
Mass rearing of temperature sensitive genetic sexing strains in the Mediterranean fruit fly (Ceratitis capitata)
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DOI：
10.1023/a:1020967810703
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发表时间：
2002-09-01
期刊：
影响因子：
1.5
通讯作者：
Caceres, C
中科院分区：
文献类型：
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作者：
Caceres, C
基于温度敏感致死（tsl）突变的遗传性别分化株系（GSS）被用于大规模不育昆虫技术（SIT）项目中产生不育雄蝇。仅使用雄性菌株提高了该技术的整体效率。目前，在世界各地的不同设施中，每周生产超过14亿只雄性不育蛹。由于构建这些菌株所使用的突变，即易位和可选择标记，它们需要与双性菌株（BSS）不同且更谨慎的大规模饲养程序。基本的饲养技术已经发展起来，可以在可预测的基础上只产生雄性，准确率达到99.9%。如果遵循特定的饲养程序，则基于tsl的GSS的饲养效率与BSS的饲养效率相等，并且已经知道基于tsl的GSS产生的雄性与BSS产生的雄性质量相等。根据目前的饲养技术，这两种菌株的雄蛹生产成本大致相同。这是由于基于tls的GSS需要大量的集群。本文讨论了在大规模饲养GSS期间需要考虑的因素，并确定了目前可用的最有效的生产工艺。
Genetic sexing strains (GSS) based on the temperature sensitive lethal (tsl) mutation are being used to produce sterile male medflies for large scale sterile insect technique (SIT) programmes for this pest. The use of male-only strains increases the overall efficiency of the technique. Currently more than 1.4 billion sterile male-only pupae are produced per week in different facilities around the world. Due to the mutations used to construct these strains, that is, translocations and selectable markers, they require different and more careful mass rearing procedures than do bisexual strains (BSS). The basic rearing technology has been developed and can be used to produce only males on a predictable basis to a level of 99.9% accuracy. If specific rearing procedures are followed, then tsl-based GSS has a rearing efficiency that is equal to that of a BSS and it is already know that males produced by the tsl-based GSS are of equal quality to males produced by BSS. Based on current rearing technology the cost of production of male pupae is about the same for both types of strain. This is due to the large colony that is required for the tsl-based GSS. This paper discusses the considerations that need to be taken into account during mass rearing of GSS and identifies the most efficient production processes that are currently available.