Pretreatment Before Ischemia Induction with Polymerized Human Placenta Hemoglobin (PolyPHb) Attenuates Ischemia/Reperfusion Injury-induced Myocardial Apoptosis
Pretreatment Before Ischemia Induction with Polymerized Human Placenta Hemoglobin (PolyPHb) Attenuates Ischemia/Reperfusion Injury-induced Myocardial Apoptosis
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DOI：
10.3109/10731199.2010.494581
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发表时间：
2011-01
期刊：
影响因子：
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通讯作者：
Wei Wu;Tao Li;Jin Liu;Chengmin Yang
中科院分区：
文献类型：
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作者：
Wei Wu;Tao Li;Jin Liu;Chengmin Yang
摘要本研究旨在观察聚合人胎盘血红蛋白（PolyPHb）预处理对缺血/再灌注（I/R）损伤诱导的心肌细胞凋亡的影响。采用Langendorff模型灌流SD大鼠心脏。基础灌流后，分别用含或不含PolyPHb的St.托马斯液（STS组）停搏心脏，然后进行I/R损伤。我们的结果表明，PolyPHb预处理大大减少了TUNEL阳性的心肌细胞，降低了caspase-3的活性和切割，这进一步证实了线性回归分析。因此，PolyPHb预处理有利于减轻心肌细胞凋亡，对心脏I/R损伤提供保护。
Abstract This study was designed to investigate the influence of polymerized human placenta hemoglobin (PolyPHb) pretreatment on ischemia/reperfusion (I/R) injury-induced myocardial apoptosis. Isolated Sprague-Dawley rat hearts were perfused by Langendorff model. After basal perfusion, hearts were arrested by St.Thomas’ solution (STS) with (Pre-HBOC group) or without PolyPHb (STS group), then subjected to I/R injury. Our results showed that PolyPHb pretreatment greatly reduced the TUNEL-positive myocardial cells and decreased the caspase-3 activity and cleavage, which was further confirmed by the linear regression analysis. Therefore, PolyPHb pretreatment was beneficial to attenuation of myocardial apoptosis and provided protection to the heart against I/R injury.