Development of pollen-induced allergic rhinitis with early and late phase nasal blockage in guinea pigs
Development of pollen-induced allergic rhinitis with early and late phase nasal blockage in guinea pigs
复制标题
豚鼠花粉诱发的过敏性鼻炎的早期和晚期鼻塞的发展
作者：
T. Nabe;N. Mizutani;K. Shimizu;H. Takenaka;S. Kohno
摘要.目的和设计：在豚鼠中评估反复吸入日本柳杉花粉挑战期间鼻塞和打喷嚏的情况。¶受试者：雄性哈特利豚鼠。¶治疗：通过鼻内滴注柳杉花粉提取物 + Al(OH)3，每天 2 次，持续 7 天，使豚鼠致敏。然后每周一次强迫动物吸入用于攻击的花粉，这些花粉被限制性地困在上呼吸道中。¶方法：在 30 次攻击后，每次都测量特定气道阻力 (sRaw) 的变化、打喷嚏频率和血清中过敏性抗体的滴度。¶结果：第一次攻击时，未观察到 sRaw 明显增加。然而，对动物的第二次和第三次挑战导致 sRaw 适度双相升高，在第一小时和第四小时至第六小时达到峰值。在第四到第十次挑战中，观察到 sRaw 显着升高。然而，随着吸入挑战的重复，早期和晚期的反应变得几乎无法区分，因为随着反应的扩大，部分重叠。所有豚鼠在每次花粉吸入挑战后立即打喷嚏。第七次攻击后，循环中的γ1和IgE抗体均明显增加。¶结论：这些结果表明，本研究建立的实验性变应性鼻炎可以成为分析该疾病发病机制和开发新治疗药物的有价值的模型。
Abstract.Objective and Design: Development of nasal blockage and sneezing during repeated inhalation challenges with Japanese cedar pollens was evaluated in guinea pigs.¶Subjects: Male Hartley guinea pigs.¶Treatment: Guinea pigs were sensitized by intranasal instillation of cedar pollen extracts + Al(OH)3 2 times a day for 7 days. The animal was then forced to inhale the pollens for challenge, which was restrictively trapped in the upper airways, once a week.¶Methods: Change of specific airway resistance (sRaw), sneezing frequency, and titers of anaphylactic antibodies in the serum were measured after each of the 30 challenges.¶Results: At the first challenge, no obvious increase in sRaw was observed. However, the second and third challenges to the animals caused modest biphasic elevations of sRaw, with peaks at the first and the fourth to sixth hour. At the fourth to tenth challenges, marked elevations of sRaw were observed. However, with repetition of the inhalation challenge, the early and the late responses became almost indistinguishable because of partial overlapping as the responses expanded. All guinea pigs sneezed immediately after each pollen inhalation challenge. Apparent increases of both circulating γ1 and IgE antibodies were seen after the seventh challenge.¶Conclusions: These results indicate that the experimental allergic rhinitis established in the present study can be a valuable model for analyzing the pathogenesis of the disease and developing new therapeutic drugs.
抗原鼻激发的早期、晚期和再激发反应之间的关系：炎症介质和细胞作用的观察。
DOI：
10.1016/s0091-6749(05)80146-0
发表时间：
1990
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Iliopoulos,O;Proud,D;AdkinsonJr,NF;Norman,PS;Kagey-Sobotka,A;Lichtenstein,LM;Naclerio,RM
通讯作者：
Naclerio,RM
短期全身糖皮质激素治疗对抗原攻击后人鼻介质释放的影响。
DOI：
10.1172/jci113188
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Pipkorn,U;Proud,D;Lichtenstein,LM;Schleimer,RP;Peters,SP;AdkinsonJr,NF;Kagey-Sobotka,A;Norman,PS;Naclerio,RM
通讯作者：
Naclerio,RM
通过吹入豚草花粉提取物诱导鼻腔晚期反应。
DOI：
10.1016/0091-6749(84)90409-3
发表时间：
1984
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Dvoracek,JE;Yunginger,JW;Kern,EB;Hyatt,RE;Gleich,GJ
通讯作者：
Gleich,GJ