The Molecular Epidemiology of Prevalent Klebsiella pneumoniae Strains and Humoral Antibody Responses against Carbapenem-Resistant K. pneumoniae Infections among Pediatric Patients in Shanghai.
The Molecular Epidemiology of Prevalent Klebsiella pneumoniae Strains and Humoral Antibody Responses against Carbapenem-Resistant K. pneumoniae Infections among Pediatric Patients in Shanghai.
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作者：
耐碳青霉烯类肺炎克雷伯菌（CRKP）在全球儿科患者中广泛传播，引起了公众的关注。本研究调查了140例无重复感染的临床克雷伯氏菌的临床流行病学特征。2021年1月至12月期间从儿科患者中分离出的肺炎菌株。在所有分离株中，16.43%（23/140）为CRKP菌株，主要含有KPC碳青霉烯酶。wzi测序结果显示，KL 47（65.22%，15/23）是最常见的荚膜型，其次是KL 64（17.39%，4/23）。共23株CRKP分为三种不同的O基因型，包括OL 101（65.22%，15/23），O 1（26.09%，6/23）和O3（8.7%，2/23）。有趣的是，KL 47菌株与OL 101紧密相关，而KL 64菌株均与O 1连锁。通过血清学分型、噬菌体分型、解聚酶分型和糖醛酸测定鉴定了部分荚膜缺陷型菌株。本研究首次在K47和K64 K患儿血清中检测到抗荚膜多糖（CPS）IgG抗体，并与健康儿童进行了比较。肺炎克雷伯氏菌感染的儿童，对相应的血清型K具有有效的杀菌活性。pneumoniae菌株这些发现将促进新的治疗和疫苗策略的开发。小儿肺炎感染。重要性碳青霉烯类耐药肺炎克雷伯菌（CRKP）菌株对多种抗生素耐药的出现以及有限的治疗选择已成为免疫功能低下儿童人群的紧迫健康威胁。疫苗和抗体，特别是针对荚膜多糖的疫苗和抗体，可能是新的有效的预防和治疗选择。因此，了解CRKP在儿科人群中的传播非常重要。本研究表明，OL 101：KL 47和O 1：KL 64是中国上海地区儿童CRKP菌株的主要组合。CRKP菌株中的主要碳青霉烯酶基因为KPC。此外，本研究发现抗K47和K64 K的抗CPS IgG水平升高。首次在儿科患者中发现肺炎菌株。证实了这些抗CPS IgG的显著杀菌活性。
Carbapenem-resistant Klebsiella pneumoniae (CRKP) has caused wide dissemination among pediatric patients globally and thus has aroused public concern. Here, we investigated the clinical epidemiological characteristics of 140 nonreplicate clinical K. pneumoniae strains isolated from pediatric patients between January and December 2021. Of all isolates, 16.43% (23 of 140) were CRKP strains, which predominantly contained KPC carbapenemase. wzi sequencing demonstrated that KL47 (65.22%, 15 of 23) was the most frequent capsular type, followed by KL64 (17.39%, 4 of 23). A total of 23 CRKP strains were classified into three different O-genotypes, including OL101 (65.22%, 15 of 23), O1 (26.09%, 6 of 23), and O3 (8.7%, 2 of 23). Interestingly, KL47 strains were strongly associated with OL101, while KL64 strains were all linked with O1. Some capsule-deficient strains were identified by serological typing, phage-typing, depolymerase-typing, and uronic acid assay. In this study, compared with healthy children, higher titers of anti-capsular polysaccharides (CPS) IgG were first detected in the sera of K47 and K64 K. pneumoniae-infected children, which had the effective bactericidal activity against corresponding serotype K. pneumoniae strains. These findings will facilitate the development of novel therapeutic and vaccine strategies against K. pneumoniae infection in children. IMPORTANCE The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains resistant to numerous antibiotics and the limited therapeutic options available have become an urgent health threat to the immunocompromised pediatric population. Vaccines and antibodies, especially those targeting capsular polysaccharides, may be novel and effective prevention and treatment options. Thus, it is important to understand the spread of CRKP in pediatric populations. This research presents OL101:KL47 and O1:KL64 as the predominant combinations among CRKP strains in children in Shanghai, China. The primary carbapenemase gene is KPC in CRKP strains. Additionally, this study found elevated levels of anti-CPS IgG against K47 and K64 K. pneumoniae strains in pediatric patients for the first time. The significant bactericidal activity of these anti-CPS IgGs was confirmed.
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DOI：
10.3390/pathogens10030279
发表时间：
2021-03-02
期刊：
Pathogens (Basel, Switzerland)
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