Selective enrichment of sialylated glycopeptides with mesoporous poly-melamine-formaldehyde (mPMF) material
Selective enrichment of sialylated glycopeptides with mesoporous poly-melamine-formaldehyde (mPMF) material
复制标题
用介孔聚三聚氰胺甲醛（mPMF）材料选择性富集唾液酸化糖肽。
DOI：
10.1007/s00216-020-02415-z
复制
发表时间：
2020-02-05
影响因子：
4.3
通讯作者：
Liang, Xinmiao
中科院分区：
文献类型：
--
作者：
Wan, Huihui;Zhang, Xiaofei;Liang, Xinmiao
由于唾液酸化糖肽(SGPS)在复杂的生物样品中的丰度相对较低，以及SGPS在质谱学中的信号较低，因此糖蛋白唾液酸化的分析具有挑战性。本研究制备了一种介孔聚三聚氰胺-甲醛(MPMF)聚合物，并将其作为SGPS的高效吸附剂。MPMF聚合物具有高比表面积(755.4 m(2)g(-1))和高密度的胺和三嗪官能团。该聚合物对SGPS具有较高的选择性(耐100摩尔倍数的牛血清白蛋白干扰)和较高的吸附容量(560 mg g(-1))。MPMF对SGPS的高性能归因于mPMF独特的物理化学性质和高密度的糖肽结合位点。进一步将mPMF应用于HeLa S3细胞裂解物，得到了576个特征糖肽，具有218个独特的糖基化位点。这一发现为蛋白质糖基化的表征提供了一种新的提取方法的选择。图形摘要合成了一种介孔聚三聚氰胺-甲醛(MPMF)聚合物，并将其用作唾液酸化糖肽(SGPS)的高效富集剂。
Analysis of glycoprotein sialylation is challenging due to the relatively low abundance of sialylated glycopeptides (SGPs) in complex biosamples and low signals of SGPs in mass spectrometry. In this study, a mesoporous poly-melamine-formaldehyde (mPMF) polymer was prepared and utilized as the high-efficiency sorbent for SGPs. The mPMF polymer featured high surface area (755.4 m(2) g(-1)) and high density of amine and triazine functional groups. This polymer demonstrated high enrichment selectivity (resistant to 100 molar fold interference of BSA) and superior adsorption capacity (560 mg g(-1)) for SGPs. The high performance of mPMF toward SGPs ascribes to the unique physicochemical properties of mPMF and high density of accessible binding sites for glycopeptides. Further application of mPMF to HeLa S3 cell lysate resulted in 576 characterized glycopeptides with 218 unique glycosylation sites. This finding provides a new choice of promising extraction approach for characterization of protein glycosylation.Graphical abstract A mesoporous poly-melamine-formaldehyde (mPMF) polymer was prepared and utilized as the high-efficiency enrichment sorbent for sialylated glycopeptides (SGPs).