Hydrogen Peroxide-Producing Lactobacilli Are Associated With Lower Levels of Vaginal Interleukin-1β, Independent of Bacterial Vaginosis.
Hydrogen Peroxide-Producing Lactobacilli Are Associated With Lower Levels of Vaginal Interleukin-1β, Independent of Bacterial Vaginosis.
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产生过氧化氢的乳杆菌与较低水平的阴道白细胞介素1β相关，与细菌性阴道病无关。
DOI：
10.1097/olq.0000000000000298
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发表时间：
2015-07
影响因子：
3.1
通讯作者：
Hitti J
中科院分区：
文献类型：
--
作者：
Mitchell C;Fredricks D;Agnew K;Hitti J
阴道中存在产生过氧化氢（H2 O2）的乳杆菌与早产率和艾滋病毒感染率的降低有关。我们推测这是由于这些物种的免疫调节作用。采用Nugent评分法对4组女性阴道拭子中IL 1 β、IL 6、IL 8、分泌性白细胞蛋白酶抑制剂（SLPI）和人β防御素2（HBD 2）的浓度进行定量分析：有和无细菌性阴道病（BV）的女性，通过半定量培养法检测产生H2 O2的乳酸杆菌进一步分层。10个定量PCR测定表征了每组中选择的乳杆菌和BV相关物种的存在和数量。使用ANOVA、Kruskal-Wallis、Mann Whitney U或卡方检验比较四组之间的免疫标志物和细菌水平。来自四组的110名妇女的拭子包括：26名具有正常的Nugent评分（BV-），未检测到产生H2 O2的乳酸杆菌（H2 O2-），47名为BV-，H2 O2+，27名BV+，H2 O2-和10名BV+，H2 O2+。两组在年龄、婚姻状况和生育史方面相似，但种族不同：BV−、H2 O2 −组有更多的白人参与者（p = 0.02）。在有和无BV的女性中，H2 O2+组的IL 1 β较低。HBD 2在BV+ H2 O2-女性中最低，在BV-，H2 O2-中最高。女性BV患者SLPI较低，且与是否存在产生H2 O2的乳酸杆菌无差异。在回归分析中，L.卷曲肌与较低的IL 1 β含量相关。通过qPCR检测BV相关物种的数量在有和没有BV的女性之间存在显著差异，但在这些组中有和没有产生H2 O2的乳酸杆菌的女性之间没有差异。产生H2 O2的乳酸杆菌的存在与某些阴道促炎细胞因子的水平较低有关，即使在BV女性中也是如此。
The presence of hydrogen peroxide (H2O2)-producing lactobacilli in the vagina is associated with decreased rates of preterm birth and HIV acquisition. We hypothesize that this is due to immunomodulatory effects of these species. Concentrations of IL1β, IL6, IL8, secretory leukocyte protease inhibitor (SLPI) and human beta defensin 2 (HBD2) were quantified from vaginal swabs from 4 groups of women: women with and without bacterial vaginosis (BV) by Nugent score, further stratified by detection of H2O2-producing lactobacilli by semi-quantitative culture. Ten quantitative PCR assays characterized presence and quantity of select Lactobacillus and BV-associated species in each group. Levels of immune markers and bacteria were compared between the four groups using ANOVA, Kruskal-Wallis, Mann Whitney U or chi-square tests. Swabs from 110 women from four groups were included: 26 had a normal Nugent score (BV−), and no H2O2-producing lactobacilli detected(H2O2−), 47 were BV−, H2O2+, 27 BV+, H2O2− and 10 BV+, H2O2+. The groups were similar in age, marital status and reproductive history, but not ethnicity: the BV−, H2O2− group had more Caucasian participants(p = 0.02). In women with and without BV, IL1β was lower in the H2O2+ groups. HBD2 was lowest in BV+ H2O2− women and highest in BV−, H2O2−. SLPI was lower in women with BV, and did not differ by the presence of H2O2–producing lactobacilli. In regression analysis higher quantities of L. crispatus were associated with lower quantities of IL1β. Detection and quantity of BV-associated species by qPCR was significantly different between women with and without BV, but not between women with and without H2O2-producing lactobacilli within those groups. The presence of H2O2-producing lactobacilli is associated with lower levels of some vaginal pro-inflammatory cytokines, even in women with BV.