A NEW DISPENSABLE GENETIC-LOCUS OF THE TERMINUS REGION INVOLVED IN CONTROL OF CELL-DIVISION IN ESCHERICHIA-COLI
A NEW DISPENSABLE GENETIC-LOCUS OF THE TERMINUS REGION INVOLVED IN CONTROL OF CELL-DIVISION IN ESCHERICHIA-COLI
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DOI：
10.1007/bf00425651
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
BOUCHE, JP
中科院分区：
文献类型：
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作者：
BEJAR, S;BOUCHE, JP
在大肠杆菌染色体的末端区域的局部诱变后，分离出细胞分裂缺陷的温度敏感突变体。在这些突变体之一，dicA，有缺陷的基因被映射在34.9分钟与manA和三个物理特征的Tn 10插入的连锁。温度敏感性所赋予的突变dicA 1在recA的背景下被抑制的存在下，携带野生型基因的杂交质粒。此外，该突变被附近基因dicB的转座子失活或整个dicA-dicB间隔的缺失所抑制。这些结果将dicA-dicB位点定义为参与细胞分裂控制的新的遗传簇。
Temperature-sensitive mutants defective in cell division were isolated after localised mutagenesis of the terminus region of the Escherichia coli chromosome. The defective gene in one of these mutants, dicA, was mapped at 34.9 min by linkage with manA and with three physically characterized Tn10 insertions. Temperature-sensitivity conferred by mutation dicA1 in a recA backround was suppressed by the presence of hybrid plasmids carrying the wild-type gene. In addition, the mutation was suppressed either by tranposon inactivation of a nearby gene, dicB, or by deletion of the entire dicA-dicB interval. These results define the dicA-dicB locus as a new dispensable genetic cluster involved in the control of cell division.