Depressive-like behavior and stress reactivity are independent traits in a Wistar Kyoto x Fisher 344 cross.
Depressive-like behavior and stress reactivity are independent traits in a Wistar Kyoto x Fisher 344 cross.
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抑郁样行为和应激反应是 Wistar京都 x Fisher 344 杂交的独立特征。
DOI：
10.1038/sj.mp.4001255
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Redei,EE
中科院分区：
文献类型：
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作者：
Solberg,LC;Ahmadiyeh,N;Baum,AE;Vitaterna,MH;Takahashi,JS;Turek,FW;Redei,EE
抑郁症是一种遗传性疾病，通常由压力引起。应激反应性下丘脑-垂体-肾上腺（HPA）轴的紊乱在抑郁症患者中也很常见。在动物模型中，强迫游泳测试（FST）是最常用的抑郁样行为测试。我们已经使用了一个建议的抑郁症动物模型，Wistar京都（WKY）大鼠，调查的关系，以及FST行为和HPA措施的遗传模式。通过WKY和F344亲本的正反交和F1代的兄妹交配，共获得F2代486头。在FST中对亲本、F1和F2代动物进行检测。采集血样测定基础和应激（10分钟约束）血浆皮质酮（CORT）水平，并测量肾上腺重量。我们发现，所有的措施都在一定程度上遗传，这种遗传性是高度依赖于性别。F2代数据的相关性和因子分析表明，FST行为和HPA轴措施没有直接关系。因此，在WKY× F344杂交的分离F2代中，抑郁样行为和HPA轴异常的潜在遗传成分可能是不同的。
Depression is a heritable disorder that is often precipitated by stress. Abnormalities of the stress-reactive hypothalamic–pituitary–adrenal (HPA) axis are also common in depressed patients. In animal models, the forced swim test (FST) is the most frequently used test of depressive-like behavior. We have used a proposed animal model of depression, the Wistar Kyoto (WKY) rat, to investigate the relationship as well as the mode of inheritance of FST behaviors and HPA measures. Through reciprocal breeding of WKY and F344 parent strains and brother–sister breeding of the F1 generation, we obtained 486 F2 animals. Parent, F1 and F2 animals were tested in the FST. Blood samples were collected for determination of basal and stress (10-min restraint) plasma corticosterone (CORT) levels, and adrenal weights were measured. We found that all measures were heritable to some extent and that this heritability was highly sex dependent. Both correlation and factor analyses of the F2 generation data demonstrate that FST behavior and HPA axis measures are not directly related. Thus, the underlying genetic components of depressive-like behavior and HPA axis abnormalities are likely to be disparate in the segregating F2 generation of a WKY× F344 cross.