Experimental Research on Wild-type p53 Plasmid Transfected into Retinoblastoma Cells and Tissues using an Ultrasound Microbubble Intensifier
Experimental Research on Wild-type p53 Plasmid Transfected into Retinoblastoma Cells and Tissues using an Ultrasound Microbubble Intensifier
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超声微泡增强器转染野生型p53质粒转染视网膜母细胞瘤细胞和组织的实验研究
DOI：
10.1177/147323001003800327
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发表时间：
2010-05-01
影响因子：
1.6
通讯作者：
Wang, Z.
中科院分区：
文献类型：
--
作者：
Luo, J.;Zhou, X.;Wang, Z.
采用超声微泡技术研究野生型p53（wtp 53）在视网膜母细胞瘤（RB）Y 79细胞中的转染效率。还使用人RB裸鼠异种移植肿瘤模型来研究该技术是否可以将wtp 53递送到实体瘤中。逆转录-聚合酶链反应（RT-PCR）结果显示，质粒加超声组和质粒加脂质体组均能成功转染Y 79细胞，而质粒加超声组和空白对照组均不能成功转染。流式细胞仪检测结果显示，微泡组和超声组的细胞凋亡率最高（25.58%），质粒+脂质体组（19.50%），其他两组（< 10%）。RT-PCR结果显示，超声组和微泡组wtp 53基因均成功转染到实体瘤中。这项研究提供了初步证据，支持RB基因治疗的潜在新方法。
The transfection efficiency of wild-type p53 (wtp53) was investigated in retinoblastoma (RB) Y79 cells using an ultrasound microbubble technique. A human RB nude mouse xenograft tumour model was also used to investigate whether this technique could deliver wtp53 into solid tumours. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that wtp53 was successfully transfected into Y79 cells in the plasmid with microbubbles and ultrasound group and in the plasmid with liposomes group, but not in the plasmid with ultrasound group or in the untreated control group. Flow cytometry showed that apoptosis was highest in the microbubbles and ultrasound group (25.58%) compared with the plasmid with liposomes group (19.50%), and the other two groups (< 10%). RT-PCR also showed that the wtp53 gene was successfully transfected into solid tumours in the plasmid with microbubbles and ultrasound group. This study provides preliminary evidence in support of a potential new approach to RB gene therapy.