Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging.

Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging.
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DOI:
10.12688/f1000research.21294.1
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发表时间:
2019-01-01
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影响因子:
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通讯作者:
Patton, Brian R
Patton, Brian R
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其他
文献类型:
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作者:
Grant, Stephen D;Cairns, Gemma S;Patton, Brian R

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我们报告了一种基于Openflexure项目设计的3D打印显微镜,该显微镜使用低成本组件来执行荧光成像。该系统具有足够的灵敏度和机械稳定性,允许使用超分辨率径向波动算法来获得分辨率优于衍射极限的图像。由于低成本的组件,整个系统可以建立约1200美元。
We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.