Macros in microRNA target identification: a comparative analysis of in silico, in vitro, and in vivo approaches to microRNA target identification.
Macros in microRNA target identification: a comparative analysis of in silico, in vitro, and in vivo approaches to microRNA target identification.
复制标题
MicroRNA靶标识别中的宏：在体外和体内方法中的MicroRNA靶标识别方法的比较分析。
DOI：
10.4161/rna.28649
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发表时间：
2014
期刊：
影响因子：
4.1
通讯作者：
Weston MD
中科院分区：
文献类型：
--
作者：
Tarang S;Weston MD
微小RNA（microRNAs，miRNAs）是一种短RNA分子，通过与靶基因上的互补序列进行部分或不完全碱基配对来调节转录后基因的表达。基于序列的miRNA靶基因识别使得能够利用计算方法，其在从基因组中识别推定的miRNA靶的子集方面具有高度的信息性。随后，通过体外测定实验评估单个miRNA-靶基因结合，以验证和量化miRNA-靶基因相互作用的转录或转录后效应。虽然离体方法在为进一步分析提供基础方面具有指导性，但体内遗传学研究对于确定生理条件下miRNA靶点的发生和生物学相关性至关重要。在本综述中，我们总结了每种实验方法的重要特征，它们的技术和生物学局限性，以及根据miRNA靶基因识别的复杂性所面临的未来挑战。
MicroRNAs (miRNAs) are short RNA molecules that modulate post-transcriptional gene expression by partial or incomplete base-pairing to the complementary sequences on their target genes. Sequence-based miRNA target gene recognition enables the utilization of computational methods, which are highly informative in identifying a subset of putative miRNA targets from the genome. Subsequently, single miRNA–target gene binding is evaluated experimentally by in vitro assays to validate and quantify the transcriptional or post-transcriptional effects of miRNA–target gene interaction. Although ex vivo approaches are instructive in providing a basis for further analyses, in vivo genetic studies are critical to determine the occurrence and biological relevance of miRNA targets under physiological conditions. In the present review, we summarize the important features of each of the experimental approaches, their technical and biological limitations, and future challenges in light of the complexity of miRNA target gene recognition.