Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria
Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria
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DOI：
10.1016/j.febslet.2006.09.020
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发表时间：
2006-10-16
期刊：
影响因子：
3.5
通讯作者：
Papa, Sergio
中科院分区：
文献类型：
--
作者：
Sardanelli, Anna Maria;Signorile, Anna;Papa, Sergio
证据表明存在于大鼠心脏线粒体内室的AKAP121和相关的PKA。免疫印迹分析和胰蛋白酶消化模式显示，无论是从分离的线粒体还是从心肌细胞培养中制备，90%或更多的线粒体C-PKA、R-PKA和AKAP121都位于线粒体内室。通过测量PKA的特异催化活性、（32）p-磷酸化的C-PKA对R-PKA的放射性标记和p- 32磷酸化的R-PKA对AKAP的放射性标记以及暴露于金偶联的AKAP121抗体的线粒体的电子显微镜，证实了这种定位。(c) 2006年欧洲生化学会联合会。Elsevier B.V.版权所有。
Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by (32)p-phosphorylated C-PKA and of AKAP by P-32-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.