ANALYSIS OF FLUORESCENCE DECAY CURVES BY MEANS OF LAPLACE TRANSFORMATION
ANALYSIS OF FLUORESCENCE DECAY CURVES BY MEANS OF LAPLACE TRANSFORMATION
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DOI：
10.1016/s0006-3495(75)85817-6
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发表时间：
1975-01-01
影响因子：
3.4
通讯作者：
BRAND, L
中科院分区：
文献类型：
--
作者：
GAFNI, A;MODLIN, RL;BRAND, L
本文描述了一种分析有限宽灯闪光卷积的荧光衰减数据的计算方法。计算机程序计算不同s值下衰减和灯闪的拉普拉斯变换之比，以给出每个s值下脉冲响应的变换。这些被设置为所涉及的衰减定律的解析拉普拉斯变换。非线性联立方程的解可得到所需的衰减参数。该方法可以修改以分析由于散射光而包含成分的数据，并且还可以提供有关光电倍增管随发射波长变化的传输时间变化的基本信息。通过对实际数据和模拟数据的分析，验证了该方法的有效性。分析的准确性取决于参数之间的相关程度。
A computational procedure is described for the analysis of fluorescence decay data convolved with a lamp flash of finite width. The computer program calculates the ratio of the Laplace transforms of the decay and the lamp flash for different values of s to give the transforms of the impulse response for each value of s. These are set equal to the analytical Laplace transforms of the decay law involved. Solution of the nonlinear simultaneous equations yields the desired decay parameters. The method can be modified to analyze data that contains a component due to scattered light and can also provide essential information regarding transit time changes of the photomultiplier with changes in emission wavelength. The method was tested by the analysis of real and simulated data. The accuracy of the analysis depends on the degree of correlation among the parameters.