Analysis of Microarray-Identified Genes and MicroRNAs Associated with Idiopathic Pulmonary Fibrosis.
Analysis of Microarray-Identified Genes and MicroRNAs Associated with Idiopathic Pulmonary Fibrosis.
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微阵列鉴定的与特发性肺纤维化相关的基因和 MicroRNA 的分析
DOI：
10.1155/2017/1804240
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发表时间：
2017
影响因子：
4.6
通讯作者：
Yi X
中科院分区：
文献类型：
--
作者：
Fan L;Yu X;Huang Z;Zheng S;Zhou Y;Lv H;Zeng Y;Xu JF;Zhu X;Yi X
本研究的目的是通过网络微阵列鉴定与特发性肺纤维化（IPF）相关的潜在microRNA和基因。微RNA微阵列数据集GSE 32538和mRNA数据集GSE 32537、GSE 53845和GSE 10667从Gene Expression Omnibus（GEO）数据库下载。用GEO 2 R筛选差异表达的miRNAs（DE-miRNAs）/基因（DEG），并通过全面的生物信息学分析来分析它们与IPF的关联。在IPF和对照组织中共鉴定出45种DE-microRNA，而在所有三种微阵列中确定了67种常见的DEG表现出相同的表达趋势。此外，功能分析表明，癌症中的microRNA和ECM-受体相互作用是最重要的途径，并被45种DE-miRNAs和67种常见的DEG富集。最后，我们通过使用至少三个在线程序预测了17个DE-miRNA和17个DEG之间潜在的microRNA-靶标相互作用。在DE-miRNAs和DEG之间构建了一个microRNA介导的调控网络，这可能为预测IPF进展的潜在生物标志物提供新的线索。
The aim of this study was to identify potential microRNAs and genes associated with idiopathic pulmonary fibrosis (IPF) through web-available microarrays. The microRNA microarray dataset GSE32538 and the mRNA datasets GSE32537, GSE53845, and GSE10667 were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed miRNAs (DE-miRNAs)/genes (DEGs) were screened with GEO2R, and their associations with IPF were analyzed by comprehensive bioinformatic analyses. A total of 45 DE-microRNAs were identified between IPF and control tissues, whereas 67 common DEGs were determined to exhibit the same expression trends in all three microarrays. Furthermore, functional analysis indicated that microRNAs in cancer and ECM-receptor interaction were the most significant pathways and were enriched by the 45 DE-miRNAs and 67 common DEGs. Finally, we predicted potential microRNA-target interactions between 17 DE-miRNAs and 17 DEGs by using at least three online programs. A microRNA-mediated regulatory network among the DE-miRNAs and DEGs was constructed that might shed new light on potential biomarkers for the prediction of IPF progression.