A microfluidic platform for measuring electrical activity across cells
A microfluidic platform for measuring electrical activity across cells
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DOI：
10.1063/1.4754599
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发表时间：
2012-09-01
期刊：
影响因子：
3.2
通讯作者：
Hua, Susan Z.
中科院分区：
文献类型：
--
作者：
Bathany, Cedric;Beahm, Derek L.;Hua, Susan Z.
在本文中，我们提出了一种微流控芯片，能够测量电导通过间隙连接通道在一个二维的细胞片。该芯片利用三股层流在两种导电溶液之间产生非导电蔗糖间隙，使得电流可以仅通过细胞穿过蔗糖间隙。使用该芯片，我们测试了间隙连接抑制剂2-APB对NRK-49 F细胞中连接蛋白43（Cx43）间隙连接通道电耦合的影响。我们发现2-APB以剂量依赖性方式可逆地阻断电导率。三流芯片还允许我们同时跟踪电导变化和染料扩散的真实的时间。我们发现，2-APB影响电导和扩散，支持的解释，这两组数据反映了相同的间隙连接活动。该芯片提供了一个通用的平台，研究间隙连接的属性，并筛选药物，可能会抑制或加强间隙连接传输。(C)2012年美国物理学会。[http：//dx.doi.org/10.1063/1.4754599]
In this paper, we present a microfluidic chip that is capable of measuring electrical conductance through gap junction channels in a 2-dimensional cell sheet. The chip utilizes a tri-stream laminar flow to create a non-conductive sucrose gap between the two conducting solutions so that electrical current can pass across the sucrose gap only through the cells. Using the chip, we tested the effect of a gap junction inhibitor, 2-APB, on the electrical coupling of connexin 43 (Cx43) gap junction channels in NRK-49F cells. We found that 2-APB reversibly blocks the conductivity in a dose-dependent manner. The tri-stream chip further allows us to simultaneously follow the conductance changes and dye diffusion in real time. We show that 2-APB affects both conductance and diffusion, supporting the interpretation that both sets of data reflect the same gap junction activity. The chip provides a generic platform to investigate gap junction properties and to screen drugs that may inhibit or potentiate gap junction transmission. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4754599]