Enhanced gametocyte formation by Plasmodium chabaudi in immature erythrocytes: Pattern of production, sequestration, and infectivity to mosquitoes
Enhanced gametocyte formation by Plasmodium chabaudi in immature erythrocytes: Pattern of production, sequestration, and infectivity to mosquitoes
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DOI：
10.2307/3284196
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发表时间：
1996-12-01
影响因子：
1.3
通讯作者：
Landau, I
中科院分区：
文献类型：
--
作者：
Gautret, P;Miltgen, F;Landau, I
本研究的目的是研究恰鲍迪疟原虫配子体的时间生物学和感染性。为了增加配子体的产生，用苯肼治疗小鼠以诱导网织红细胞增多。作者观察到配子细胞发生的重要刺激。接种后第二天即可看到配子体，并在每次分裂时定期产生，每 24 小时一次。配子体发育周期持续60小时，由4个连续阶段组成：36小时的0阶段，裂殖子入侵，42小时的围攻I，48小时的II阶段，54小时的III阶段。 II 期的重要部分被隔离在周围的小毛细血管中。用苯肼处理的小鼠喂养的蚊子中的卵囊数量比对照组的要多。当蚊子在一天中的不同时间被喂食时，卵囊计数的昼夜节律差异不具有统计学意义。然而，第二阶段被认为可能是最具传染性的阶段，因为与其他鼠类疟疾物种的传染性配子体阶段一样，它被隔离在外周毛细血管中。与温氏疟原虫不同，在感染阶段隔离时没有感染性高峰；这可能是由于此时发生的分裂生殖的抑制作用。
The objective of this study was to investigate the chronobiology and infectivity of the gametocytes of Plasmodium chabaudi chabaudi. In order to increase the production of gametocytes, mice were treated with phenylhydrazine to induce a hyper-reticulocytosis. The authors observed an important stimulation of gametocytogenesis. Gametocytes were seen as soon as the second day postinoculation and were produced periodically at each schizogony, every 24 hr. The gametocytic developmental cycle lasted 60 hr and consisted of 4 successive stages: stage 0 at 36 hr, from merozoite invasion, siege I at 42 hr, stage II at 48 hr, and stage III at 54 hr. An important fraction of stage II was sequestered in small peripheral capillaries. The numbers of oocysts in the mosquitoes fed on phenylhydrazine-treated mice were larger than in controls. When mosquitoes were fed at different times of the day, circadian differences in the oocyst counts were not statistically significant. However, stage II was considered to be probably the most infective stage because, like the infective gametocyte stage of other species of murine malaria, it is sequestered in the peripheral capillaries. In contrast with Plasmodium vinckei, there is no peak of infectivity at the time of sequestration of the infective stage; this is probably due to the inhibitory effect of the schizogony occurring at this time.