Presence and distribution of FMRFamide-like immunoreactivity in the sea cucumber Holothuria scabra (Jaeger, 1833)
Presence and distribution of FMRFamide-like immunoreactivity in the sea cucumber Holothuria scabra (Jaeger, 1833)
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DOI：
10.1007/s00435-013-0186-3
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发表时间：
2013-09-01
期刊：
影响因子：
1
通讯作者：
Withyachumnarnkul, Boonsirm
中科院分区：
文献类型：
--
作者：
Ajayi, Abayomi;Withyachumnarnkul, Boonsirm
自从Price和Greenberg（Science 197：670-671，1977）在软体动物Macrocalista nimbosa（Lightfoot，1786）中发现FMRFamide（一种心脏活性神经肽）以来，我们对FMRFamide和FMRFamide相关肽（FaRP）在门中的分布和多样性的理解稳步增加。海参Holothuria scabra（Jaeger，1833）（棘皮动物门：海参总科）是一种高价值的热带经济物种，在全球范围内被过度开发，在某些地区可能濒临灭绝。然而，由于对其生物学（包括神经激素系统）的了解有限，对该物种的保护及其对文化的适应性受到阻碍。利用间接免疫荧光技术和反相高效液相色谱法，我们证明了FMRFamide样免疫反应性在H. scabra，包括体腔液，这表明FaRPs可能在这个物种中具有神经激素活性。
Since the discovery of FMRFamide, a cardioactive neuropeptide in the mollusk Macrocallista nimbosa (Lightfoot, 1786) by Price and Greenberg (Science 197:670-671, 1977), there has been a steady increase in our understanding of the distribution and diversity of FMRFamide and FMRFamide-related peptides (FaRPs) across phyla. The sea cucumber Holothuria scabra (Jaeger, 1833) (Echinodermata: Holothuroidea) is a high-premium tropical economic species that is overexploited globally and may be in imminent danger of extinction in some areas. Conservation of this species and its amenability to culture is however hampered by the limited knowledge about its biology, including the neurohormonal system. Using indirect immunofluorescence technique with antisera raised against the tetrapeptide FMRFamide as well as reverse-phase HPLC, we have demonstrated the widespread distribution of FMRFamide-like immunoreactivity in the tissues of H. scabra, including the coelomic fluid, suggesting that FaRPs might have neurohormonal activities in this species.