Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens
Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens
复制标题
DOI：
10.1111/j.1472-765x.2006.01939.x
复制
发表时间：
2006-08-01
影响因子：
2.4
通讯作者：
Sivasithamparam, K.
中科院分区：
文献类型：
--
作者：
Vinale, F.;Marra, R.;Sivasithamparam, K.
目的：哈茨木霉(Trichoderma Harzianum)菌株T22和T39是广泛应用于大田和温室作物中的两种微生物，广泛应用于多种商品生物农药和生物肥料中。方法和结果：在菌株T22产生的三种主要代谢产物中，有一种是新的氮杂环丙酮类化合物，在体外对立枯丝核菌、最终腐霉和禾谷盖曼霉菌有明显的抑制作用。小黑麦。反过来，菌株T39的滤液被证明含有两种以前从其他哈茨木霉菌株中分离出来的化合物和一种新的丁烯内酯。结论：本文报道了两株哈茨链霉菌培养滤液中主要次生代谢物的分离和性质，以及它们在与病原菌拮抗作用中的产生。本研究的意义和影响：这是商业应用菌株T22和T39产生次生代谢物的首次工作。我们的结果提供了对这些真菌代谢的更好的了解，这些真菌在生物防治中被广泛用作生物杀虫剂和/或生物肥料。
Aims: Trichoderma harzianum strains T22 and T39 are two micro-organisms used as active agents in a variety of commercial biopesticides and biofertilizers and widely applied amongst field and greenhouse crops. The production, isolation, biological and chemical characterization of the main secondary metabolites produced by these strains are investigated.Methods and Results: Of the three major compounds produced by strain T22, one is a new azaphilone that shows marked in vitro inhibition of Rhizoctonia solani, Pythium ultimum and Gaeumannomyces graminis var. tritici. In turn, filtrates from strain T39 were demonstrated to contain two compounds previously isolated from other T. harzianum strains and a new butenolide. The production of the isolated metabolites was also monitored by liquid chromatography/mass spectrometry during in vitro interaction with R. solani.Conclusions: This paper reports the isolation and characterization of the main secondary metabolites obtained from culture filtrates of two T. harzianum strains and their production during antagonistic interaction with the pathogen R. solani.Significance and Impact of the Study: This is the first work on secondary metabolites produced by the commercially applied strains T22 and T39. Our results provide a better understanding of the metabolism of these fungi, which are both widely used as biopesticides and/or biofertilizers in biocontrol.