Calibration of flow cytometry detector systems.
Calibration of flow cytometry detector systems.
复制标题
流式细胞术检测器系统的校准。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
R. Durand
中科院分区：
文献类型：
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作者：
R. Durand
描述了一种确定荧光信号的相对强度的方法，所述荧光信号由荧光激活细胞分选仪测量的荧光激活信号测量。校准过程是一个多步骤程序，其中首先评估放大器模块的信号增益，然后确定光电倍增管响应作为每个感兴趣的荧光染料和滤波器组合的管电压的函数。使用这些值，一个简单的数学计算，然后确定荧光信号的相对强度，即使样品的强度相差几个数量级。
A method of determining the relative intensity of the fluorescence signal measured by a fluorescence activated signal measured by a fluorescence activated cell sorter is described. The calibration process is a multistep procedure, in which the signal gain due to the amplifier module is first evaluated, followed by determination of the photomultiplier response as a function of tube voltage for each fluorochrome and filter combination of interest. Using these values, a simple mathematical calculation then determines the relative intensity of a fluorescent signal, even for samples differing in intensity by several orders of magnitude.