Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae
Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae
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DOI：
10.1016/j.biortech.2008.11.047
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发表时间：
2009-04-01
影响因子：
11.4
通讯作者：
Sawayama, Shigeki
中科院分区：
文献类型：
--
作者：
Matsushika, Akinori;Inoue, Hiroyuki;Sawayama, Shigeki
本研究比较了5株重组酿酒酵母的木糖发酵能力。通过使用工业菌株MA-R4发现最有效的木糖至乙醇发酵，其中来自树干毕赤酵母的木糖还原酶和木糖醇脱氢酶的基因沿着内源木酮糖激酶基因通过絮状酵母菌株IR-2的染色体整合表达。MA-R4菌株以低木糖醇产率将木糖快速转化为乙醇。此外，MA-R4菌株具有最高的乙醇产量时，发酵不仅是葡萄糖和木糖的混合物，而且混合糖的脱毒水解产物的木屑。这些结果共同表明，MA-R4可能是一个合适的重组菌株，用于进一步研究从木质纤维素水解产物中存在的混合糖大规模生产乙醇。(c)2008爱思唯尔有限公司保留所有权利。
In this study, five recombinant Saccharomyces cerevisiae strains were compared for their xylose-fermenting ability. The most efficient xylose-to-ethanol fermentation was found by using the industrial strain MA-R4, in which the genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis along with an endogenous xylulokinase gene were expressed by chromosomal integration of the flocculent yeast strain IR-2. The MA-R4 strain rapidly converted xylose to ethanol with a low xylitol yield. Furthermore, the MA-R4 strain had the highest ethanol production when fermenting not only a mixture of glucose and xylose, but also mixed sugars in the detoxified hydrolysate of wood chips. These results collectively suggest that MA-R4 may be a suitable recombinant strain for further study into large-scale ethanol production from mixed sugars present in lignocellulosic hydrolysates. (c) 2008 Elsevier Ltd. All rights reserved.