Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya.
Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya.
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DOI：
10.3390/ijms22020916
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发表时间：
2021-01-18
影响因子：
5.6
通讯作者：
Zhu X
中科院分区：
文献类型：
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作者：
Zheng S;Hao Y;Fan S;Cai J;Chen W;Li X;Zhu X
1-甲基环丙烯（1-MCP）处理是一种有效的保鲜技术，但1-MCP处理不当会导致木瓜果实成熟障碍（橡胶质地）。本研究通过代谢组学和转录组学的结合分析，揭示了1-MCP不合适导致木瓜成熟障碍的可能机制。在代谢组学分析中，共鉴定出203种差异累积代谢物（dam）。对照（CK）和1-MCP 2 h组只鉴定出24个dam，它们主要是黄酮类化合物。在对照1-MCP处理16 h和对照2 - mcp处理16 h组分别鉴定出90个和89个坝，表明长期处理1-MCP严重改变了果实成熟过程中的代谢物。1-MCP处理16 h严重减少了代谢物的数量，代谢物主要包括黄酮类、脂类、酚酸、生物碱和有机酸。RNA-Seq和代谢组学的综合分析表明，长期使用1-MCP可减少三羧酸循环的各种能量代谢物，其中乙醇酸循环受到的影响最为显著，苯丙烷途径也受到影响。这些结果为果实质量控制提供了有价值的信息，并对1-MCP处理不当引起的木瓜成熟障碍有了新的认识。
Treatment with 1-methylcyclopropylene (1-MCP) is an effective technique to preserve fruits, but inappropriate treatment with 1-MCP causes a ripening disorder (rubbery texture) in papaya fruit. In this study, a combined metabolomic and transcriptomic analysis was conducted to reveal the possible mechanism of the ripening disorder caused by unsuitable 1-MCP in papaya. A total of 203 differential accumulated metabolites (DAMs) were identified in the metabolome analysis. Only 24 DAMs were identified in the control (CK) vs. the 1-MCP 2 h group, and they were primarily flavonoids. Ninety and 89 DAMs were identified in the CK vs. 1-MCP 16 h and 1-MCP 2 h vs. 1-MCP 16 h groups, respectively, indicating that long-term 1-MCP treatment severely altered the metabolites during fruit ripening. 1-MCP 16 h treatment severely reduced the number of metabolites, which primarily consisted of flavonoids, lipids, phenolic acids, alkaloids, and organic acids. An integrated analysis of RNA-Seq and metabolomics showed that various energy metabolites for the tricarboxylic acid cycle were reduced by long-term treatment with 1-MCP, and the glycolic acid cycle was the most significantly affected, as well as the phenylpropane pathway. These results provide valuable information for fruit quality control and new insight into the ripening disorder caused by unsuitable treatment with 1-MCP in papaya.