INHIBITION OF CHORION EXPANSION AND PRESERVATION OF FERTILITY IN GOLDFISH (CARASSIUS-AURATUS) EGGS BY PROTEASE INHIBITORS
INHIBITION OF CHORION EXPANSION AND PRESERVATION OF FERTILITY IN GOLDFISH (CARASSIUS-AURATUS) EGGS BY PROTEASE INHIBITORS
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DOI：
10.1139/f93-107
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发表时间：
1993-05-01
影响因子：
2.4
通讯作者：
GOETZ, FW
中科院分区：
文献类型：
--
作者：
HSU, SY;GOETZ, FW
研究了蛋白酶抑制剂对金鱼（Carassius auratus）卵绒毛膜扩张和受精能力的体外影响。当排卵卵保存在金鱼林格溶液中时，丝氨酸蛋白酶抑制剂苯甲脒和大豆胰蛋白酶抑制剂会阻止卵子绒毛膜的扩张。绒毛膜扩张受到抑制的鸡蛋在该溶液中保留其生育能力 30 分钟。相比之下，即使存在蛋白酶抑制剂，排卵的卵子在淡水中也会失去生育能力。结果表明，水激活金鱼卵的绒毛膜扩张和生育力丧失可能涉及蛋白水解，而蛋白水解被苯甲脒和大豆胰蛋白酶抑制剂阻断。此外，阻断绒毛膜扩张可能对于保留在含有大豆胰蛋白酶抑制剂的林格氏溶液中的卵子的生育力至关重要。该解决方案的开发提供了一种将排卵的金鱼卵暂时保持在雌性体外的可行状态的方法，并将允许对该物种的受精进行进一步研究。
The in vitro effects of protease inhibitors on chorion expansion and the fertility of goldfish (Carassius auratus) eggs were investigated. Expansion of the chorion in ovulated eggs was blocked by the serine protease inhibitors benzamidine and soybean trypsin inhibitor when they were held in a goldfish Ringer solution. Eggs in which chorion expansion was inhibited retained their fertility for 30 min when held in this solution. In contrast, ovulated eggs lost their fertility in freshwater even in the presence of a protease inhibitor. The results suggest that chorion expansion and loss of fertility in water-activated goldfish eggs may involve proteolysis which was blocked by benzamidine and soybean trypsin inhibitor. Moreover, the blockage of chorion expansion may be pivotal to the preservation of fertility in eggs held in Ringer's solution with soybean trypsin inhibitor. The development of this solution provides a means to temporarily hold ovulated goldfish eggs in a viable state outside the female and will permit further studies on fertilization in this species.