Free light chains of immunoglobulins in normal serum and urine determined by radioimmunoassay.
Free light chains of immunoglobulins in normal serum and urine determined by radioimmunoassay.
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放射免疫分析法测定正常血清和尿液中免疫球蛋白的游离轻链。
DOI：
10.1080/00365517509095760
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发表时间：
1975
影响因子：
2.1
通讯作者：
K. Söulling
中科院分区：
文献类型：
--
作者：
K. Söulling
描述了用于测定血清和尿液中人免疫球蛋白的游离轻多肽链的放射免疫测定法。通过在 Sephadex G-100 柱上进行凝胶过滤，从常规免疫球蛋白中分离出游离轻链。在放射免疫测定中，通过Sephadex G-200 柱通过凝胶过滤分离游离的和抗体结合的125I标记的轻链。 20 名正常受试者血清中游离轻链的平均浓度，λ 链为 10.6 mg/1，κ 链为 13.2 mg/1。 24 小时尿中 lambda 链排泄量为 1.1 mg，kappa 链为 3.2 mg。
A radioimmunoassay for determination of free light polypeptide chains from human immunoglobulins in serum and urine is described. The free light chains were isolated from the regular immunoglobulins by gel filtration on Sephadex G-100 columns. In the radioimmunoassay the free and antibody-bound 125I-labelled light chains were separated on gel filtration through Sephadex G-200 columns. The mean concentration of free light chains in serum from 20 normal subjects was 10.6 mg/1 for lambda chains and 13.2 mg/1 for kappa chains. The 24-hr urinary excretion was 1.1 mg for lambda chains and 3.2 mg for kappa chains.