EXPRESSION OF SMALL HEAT-SHOCK PROTEINS BY THE 3RD-STAGE LARVA OF BRUGIA-PAHANGI
EXPRESSION OF SMALL HEAT-SHOCK PROTEINS BY THE 3RD-STAGE LARVA OF BRUGIA-PAHANGI
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DOI：
10.1016/0166-6851(92)90171-f
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发表时间：
1992-12-01
影响因子：
1.5
通讯作者：
DEVANEY, E
中科院分区：
文献类型：
--
作者：
JECOCK, RM;DEVANEY, E
由感染性的第三阶段幼虫（L3）的丝虫pahangi的蛋白质合成的变化进行了检查相对于温度变化所遇到的寄生虫，因为它从昆虫迁移到哺乳动物，和血清的存在下，在培养基中。虽然许多多肽的合成在体外由L3从28 ℃到37 ℃的温度变化调节，但没有证据表明血清对蛋白质合成有任何显著影响。仅通过转移至37 ℃的L3在有限的时间内合成两种小酸性多肽（22-24 kDa和18 kDa）的复合物。每个复合物的一个组分似乎在28 ℃下组成型表达，但其合成在37 ℃下上调，而每个复合物的其余成员仅在37 ℃下合成。L3和感染后（p.i.）L3的热休克（41 ℃）也诱导两个复合物的合成，这表明这些热诱导多肽与小的热休克蛋白家族有关。热休克相关蛋白在这一重要的环境转变中可能发挥的作用被认为是。
Changes in proteins synthesised by the infective third-stage larvae (L3) of the filarial nematode Brugia pahangi were examined with respect to the temperature shift encountered by the parasite as it migrates from insect to mammal, and the presence of serum in the culture medium. While the synthesis of a number of polypeptides is regulated by the temperature shift of the L3 from 28-degrees-C to 37-degrees-C in vitro, there is no evidence that serum has any significant effect on protein synthesis. Two complexes of small acidic polypeptides (22-24 kDa and 18 kDa) are synthesised for a limited period only by L3 transferred to 37-degrees-C. One component of each complex appears to be constitutively expressed at 28-degrees-C, but its synthesis is up-regulated at 37-degrees-C, while the remaining members of each complex are synthesised only at 37-degrees-C. Subjection of L3 and post-infective (p.i.) L3 to heat shock (41-degrees-C) also induces synthesis of both complexes, indicating that these heat-inducible polypeptides are related to the family of small heat shock proteins. The possible role of the heat shock-related proteins in this important environmental transition is considered.