Site specificity of mutations arising in dysgenic hybrids of Drosophila melanogaster.
Site specificity of mutations arising in dysgenic hybrids of Drosophila melanogaster.
复制标题
黑腹果蝇基因缺陷杂种中产生的突变的位点特异性。
DOI：
10.1073/pnas.77.10.6042
复制
发表时间：
1980
影响因子：
11.1
通讯作者：
Lim,JK
中科院分区：
文献类型：
--
作者：
Simmons,MJ;Lim,JK
在黑腹果蝇的非基因杂交中产生的突变被收集在X染色体的zeste-white区域。绝大多数突变影响zw 1位点;其中许多与结构异常相关，包括多线染色体图谱3A 3 -4区域的倒位、缺陷和插入。zw 1基因座的突变活性的异常杂交的极端敏感性与zw 2基因座的明显不敏感性形成对比。在zeste-white区域的其他位点对突变子活性弱敏感。两个和六个带的插入被认为是在带3A 4和3A 6之间的一个zw 1突变体股票检查的染色体。另一个插入在不同的储备液中的位置2F 4 -5处检测到。根据X染色体其他部分的继发性断裂判断，许多突变体染色体明显不稳定。细胞遗传学的数据是一致的想法，即突变引起的异常杂交是由转座因子插入优先在某些位点上的染色体。
Mutations arising in dysgenic hybrids of Drosophila melanogaster were collected in the zeste-white region of the X chromosome. A preponderance of the mutations affected the zw1 locus; many of these were asociated with structural abnormalities including inversions, deficiencies, and insertions in the 3A3-4 region of the polytene chromosome map. The extreme sensitivity of the zw1 locus to the mutator activity of dysgenic hybrids contrasted with the apparent insensitivity of the zw2 locus. Other loci in the zeste-white region were weakly sensitive to the mutator activity. Insertions of two and six bands were seen between bands 3A4 and 3A6 in the chromosomes of one of the zw1 mutant stocks examined. Another insertion was detected at position 2F4-5 in a different stock. Many of the mutant chromosome were evidently unstable, as judged by secondary breakage in other parts of the X chromosome. The cytogenetic data are consistent with the idea that mutations arising in dysgenic hybrids are caused by transposable elements which insert preferentially at certain sites on the chromosomes.