Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome
Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome
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DOI：
10.1021/acs.jproteome.9b00599
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发表时间：
2020-01-01
影响因子：
4.4
通讯作者：
Coulombe, Benoit
中科院分区：
文献类型：
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作者：
Cloutier, Philippe;Poitras, Christian;Coulombe, Benoit
PAQosome是一种由11个亚基组成的伴侣蛋白，参与多种人类蛋白质复合体的生物发生。我们发现ASDURF是最近在ASNSD1mRNA的5‘非编码区发现的一个上游开放阅读框(UORF)，它编码PAQosome的第12个亚基。ASDURF与β-前折叠蛋白具有显著的结构同源性，并与PAQosome的前折叠素样模块的5个已知亚基组装形成异六聚体前折叠素样复合体。提出了一个PAQosome类前折叠蛋白模块的模型。这些数据提供了一个真核生物uORF编码多肽的例子，其功能不仅限于顺式作用的下游编码序列的翻译调节，并强调了在蛋白质组学研究中包括替代ORF产物的重要性。
The PAQosome is an 11-subunit chaperone involved in the biogenesis of several human protein complexes. We show that ASDURF, a recently discovered upstream open reading frame (uORF) in the 5' UTR of ASNSD1 mRNA, encodes the 12th subunit of the PAQosome. ASDURF displays significant structural homology to beta-prefoldins and assembles with the five known subunits of the prefoldin-like module of the PAQosome to form a heterohexameric prefoldin-like complex. A model of the PAQosome prefoldin-like module is presented. The data presented here provide an example of a eukaryotic uORF-encoded polypeptide whose function is not limited to cis-acting translational regulation of downstream coding sequence and highlights the importance of including alternative ORF products in proteomic studies.