The formation of immune complexes is involved in the acute phase of periodontal destruction in rats
The formation of immune complexes is involved in the acute phase of periodontal destruction in rats
复制标题
免疫复合物的形成参与大鼠牙周破坏的急性期
DOI：
10.1111/j.1600-0765.2011.01453.x
复制
发表时间：
2012
影响因子：
3.5
通讯作者：
Kuramoto A
中科院分区：
文献类型：
--
作者：
K Hasegawa-Nakamura;F Tateishi;et.al.;石川 真琴;両角俊哉;福田隆男;Kuramoto A
李文，李文，李文.免疫复合物的形成参与了大鼠牙周破坏的急性期。J Periodont Res 2012; 47：455-462.© 2012 John Wiley & Sons A/S背景和目的：临床附着丧失和牙槽骨破坏是牙周炎的主要症状，不仅由牙周致病菌的破坏作用引起，还由宿主免疫系统对牙周病原体的过度反应引起。免疫系统参与牙周炎发病和进展的细节尚不清楚。在这项研究中，我们试图确定是否宿主的免疫系统，特别是免疫复合物的形成，参与牙周destruction.Material和方法：我们应用卵清蛋白或脂多糖（LPS）作为抗原和从大鼠血清中纯化的特异性免疫球蛋白G（IgG）抗体，大鼠牙龈沟交替。 通过组织学方法观察牙周组织中炎性细胞浸润和牙槽骨表面破骨细胞的数量。结果：实验组与对照组均见免疫复合物的形成，免疫复合物的形成与补体C1 qB的表达有关。用LPS和抗LPS IgG处理的组显示出更大的附着丧失。在两个实验组中，牙周组织中的炎性细胞的数量增加，而在牙槽骨嵴处的破骨细胞仅在用LPS和抗LPS IgG处理的组中观察到。结论：在本研究中，我们表明免疫复合物的形成似乎参与了牙周破坏的急性期，抗原的生物活性也很重要。
Kuramoto A, Yoshinaga Y, Kaneko T, Ukai T, Shiraishi C, Oshino K, Ichimura I, Hara Y. The formation of immune complexes is involved in the acute phase of periodontal destruction in rats. J Periodont Res 2012; 47: 455–462. © 2012 John Wiley & Sons A/SBackground and Objective:Loss of clinical attachment and alveolar bone destruction are major symptoms of periodontitis, caused by not only the destructive effect of periodontopathic bacteria but also the overactive response of the host immune system against periodontal pathogens. The details of the participation of the immune system in the onset and progression of periodontitis are unclear. In this study, we attempted to determine whether the host immune system, and in particular the formation of immune complexes, is involved in the periodontal destruction.Material and Methods:We applied ovalbumin or lipopolysaccharide (LPS) as antigens and their specific immunoglobulin G (IgG) antibodies purified from rat serum to rat gingival sulcus alternately. Loss of attachment, alveolar bone destruction and the numbers of inflammatory cells infiltrating the periodontal tissue and osteoclasts on the alveolar bone surface were investigated histometrically. The formation of immune complex was confirmed by immunohistological staining of complement C1qB.Results:Loss of attachment and the presence of C1qB were observed histopathologically in both experimental groups. The group that had been treated with LPS and anti‐LPS IgG showed greater loss of attachment. The number of inflammatory cells in the periodontal tissue was increased in both experimental groups, while osteoclasts at the alveolar bone crest were observed only in the group that had been treated with LPS and anti‐LPS IgG.Conclusion:In the present study, we showed that the formation of immune complex appears to be involved in the acute phase of periodontal destruction and that the biological activity of antigens is also important.